exons

Long-read Sequencing Can Identify RNA Isoforms and NMD Modulators

Evangelos Karousis is a Senior Researcher at the University of Bern, Switzerland and presented at the Nanopore Community Meeting 2021 on “Nanopore sequencing reveals endogenous NMD-targeted isoforms in human cells.” They began by describing what nonsense-mediated mRNA decay (NMD) can do how how they can be dangerous. Endogenous mRNAs can be targeted by NMD. In […]
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Liftoff Annotation of Novel Genes from T2T Human Genome

Alaina Shumate from John Hopkins University presented at the Nanopore Community Meeting 2021 about “The annotation of novel genes in a complete human genome.” They began by describing how in 2003 scientists “finished” the Human genome Project but there still was missing sequence! In 2021, the Telomere-to-Telomere (T2T) Consortium actually completed the sequence of a […]
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Nanopore Sequencing to Investigate Splicing Order and Deadenylation Trends

Karine Choquet from Harvard Medical School spoke at the Nanopore Community Meeting 2022 about “Direct RNA sequencing reveals multi-intron splicing order and poly(A) tail lengths across subcellular compartments.” This five-minute session began by explaining RNA processing and removal of introns in eukaryotic gene expression. Choquet explained that “RNA processing is essential for eukaryotic gene expression” […]
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