plasmids

Advancements in Bacterial and Plasmid Sequencing Techniques

Katherine Lawrence, from the Machine Learning and Bioinformatics team at Oxford Nanopore Technologies, spoke at the Nanopore Community Meeting on “Bacterial isolate and plasmid sequencing.” Lawrence began by emphasizing the importance of microbes and learning about their sequences. Long reads help assemble bacterial genomes efficiently. Bacterial genome modifications include 4mC, 5mC, and 6mA. Lawrence noted […]
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Maximize Nanopore Basecalling with Dorado: Latest Updates and Features

I want to learn how to fully utilize Dorado base calling features. Tonight, I watched Susie Lee, Technical Product Manager for Basecalling and Models at Oxford Nanopore Technologies, present the latest updates to Dorado at London Calling 2024. They explained Dorado as a software tool that “powers Nanopore basecalling and more” by taking raw signal […]
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Databases and Sequencing to Reveal the Microbiome of Wastewater Treatment Plants

Tong Zhang from The University of Hong Kong presented at London Calling 2019 on “Mobile antibiotic resistome in wastewater treatment plants revealed by nanopore metagenomic sequencing.” Zhang explained that the work was mainly performed by a student in their research group. The impact of environmental resistance was emphasized with the prevalence of antibiotic resistance genes […]
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AMRs in Complex Environmental Matrices

Rob James from the University of Warwick in the UK presented a session titled “From amplicons to metagenomes: long-read sequencing the environment” at London Calling 2019. James spoke about complex environmental matrices such as soil, sediment, and excreta that are often impure and biologically and chemically contaminated. Different extraction methods are used depending on the […]
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EPI2ME Labs Metagenomics Workflows on the Cloud

Tonight I watched the London Calling 2023 product demo for “Analysing bacterial genomes – from species identification to AMR.” Stephen Rudd, Director of Bioinformatics Product at Oxford Nanopore Technologies (ONT), shared their work on EPI2ME labs products. They have been developing EPI2ME Labs workflows for several years. These are pre-installed workflows that can help solve […]
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Whole Plasmid Nanopore Sequencing

Tonight I watched the “Plasmid sequencing, assembly and analysis workflow” video with speaker Jon Pugh, Associate Director of Platform Marketing at Oxford Nanopore Technologies. The whole plasmid nanopore sequencing workflow allows for sequencing analysis of inserts, promoters, and backbones. With Nanopore sequencing, it is possible to use a single technique “in-house.” The workflow they suggest […]
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