Year: 2023

Ultradeep Targeted Transcript Profiling!

Rosemary Bamford from the University of Exeter, UK, presented at London Calling 2022 on “Long-read transcriptome sequencing reveals isoform diversity across human neurodevelopment and aging.” Bamford spoke about how they are using long-read transcriptome sequencing to study neuropsychiatric disorders. I didn’t know neuropsychiatric disorders have such prevalence globally! There are some findings revealing genetic risk […]
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Applying Nanopore Technologies in the Undergraduate Lab

Ashley Beck from Carroll College spoke about “Applying nanopore sequencing in the undergraduate classroom for environmental microbiome analysis” at the 2022 London Calling Conference. They explained that they are dedicated to bringing technology and education to teach undergraduates. They took samples from a contaminated river from Western Montana. With students, Beck collected samples. The next […]
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Nanopore Sockeye

What is Nanopore Sockeye? Tonight I watched a session with Sockeye in the title and may finally learn what it does! Eoghan Harrington, a genomic applications fellow at Oxford Nanopore Technologies, presented at London Calling 2022 on “Sockeye: nanopore-only demultiplexing of single-cell reads.” They explained that there has been an explosion of single-cell applications and […]
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Dorado Mod-Basecaller and Visualization Tools

I have been interested in using nanopore sequencing to identify modified bases. Tonight, I watched Adrien Leger, a Senior Research Scientist at the Sequencing Chemistry program in Oxford Nanopore Technologies present at London Calling 2022. This session was an update of the Remora epi-basecaller release. They explained the similarities between remora fish and the remora […]
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Adapting Technologies with Adaptive Sampling for Target Enrichment

Abderaouf Hamza from the Institut Curie in France spoke at the Nanopore London Calling event last year about “Targeted nanopore sequencing ushers in the era of routine long-read sequencing in translational research laboratories.” This ten-minute session started with how Hamza uses nanopore sequencing for cancer genomics, particularly using adaptive sampling. They use target region enrichment […]
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Analysis of Multi-resistant Spirochaetes

Nick Vereecke from PathoSense BV Belgium presented at London Calling 2022 on “Emergence of new multi-resistant Brachyspira hyodysenteriae strains in Belgian pigs, revealed by high-quality whole-genome sequencing. This five minute session began with an introduction about the losses due to B. hyodysenteriae and swine dysentery. Vereecke noted that this organism is a spirochete bacterium with […]
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Trapping AMR Exchanges

Perfect timing! Tonight I watched Richard Goodman’s London Calling 2022 session titled “The bacterial black market: utilising sequencing to study the inter-cellular and intra-cellular transfer of AMR genes in bacteria.” I have been working on an AMR detection protocol using digital PCR for class today. Goodman is at the Liverpool School of Tropical Medicine in […]
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Creating Field Laboratories for Conservation Efforts

Tonight I watched a five-minute Oxford Nanopore Technologies London Calling 2022 session by Mrinalini Erkenswick Watsa and titled “Genomics in the jungle: a field laboratory success story.” Mrinalini Erkenswick Watsa is at the San Diego Zoo Wildlife Alliance and is a field scientist working on conservation. They spoke about the need to assess populations pre […]
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Zooming In and Out of Multi-Omics

Dan Turner leads the Applications Team at Oxford Nanopore Technologies and presented at London Calling 2022 a session entitled “A single platform for multi-omics.” Turner mentioned that they have developed a set of posters for different applications. They spoke about epithelial to mesenchymal transition (EMT). They are able to analyze this process using Nanopore sequencing […]
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