Month: August 2024

iDTECT Integration Site Analysis Assay: Revolutionizing Quality Control for Rare Diseases

Jose-Crispin Zavala Alvarado works at PathoQuest in France. They gave a presentation at London Calling 2024. The topic was “iDTECT Integration Site Analysis assay: a paradigm shift for the quality control of biologics in rare diseases.” This collaboration between PathoQuest and Oxford Nanopore was highly productive. It resulted in the launch of the first ONT-driven […]
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EPI2ME 2024: Data Analysis and Genomic Tools

Jimmy Creith is a Field Applications Scientist in bioinformatics. They work with Oxford Nanopore Technologies and presented the London Calling 2024 Masterclass I watched tonight. The session title was “How to analyse your data with EPI2ME.” We will use EPI2ME in the course. Creith focused on how EPI2ME can be used for small and large […]
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Nanopore Sequencing Library Prep: Techniques and Comparison

Tonight, I watched Jessica Anderson, a Senior Field Applications Scientist with Oxford Nanopore Technologies (ONT), present at London Calling 2024. The session was part of the Masterclass 2024 series. It was titled “How to select the right library prep workflow for your experiment.” This session started with an introduction describing how nanopore sensing works. Anderson explained that during […]
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Oxford Nanopore Technologies Masterclass: DNA and RNA Extraction Recommendations

We start the Portable Genome Sequencing course tomorrow! I wanted to review the latest Oxford Nanopore Technologies (ONT) recommendations for extracting DNA and RNA. The London Calling 2024 session “How to extract high-quality DNA and RNA” was the one I watched tonight. Vania Costa, a Field Applications Scientist with ONT, presented this ONT Masterclass. This […]
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Nanopore Sequencing: Experiment Planning and Workflow Breakdown

As we prepare for the start of a new semester, I decided to watch a Masterclass from London Calling 2024. Akelia Wauchope-Odumbo, Associate Director Technical Applications—Americas with Oxford Nanopore Technologies, presented on experimental design. The session title was “How to get started with nanopore sequencing and plan your experiment.” The masterclass series guides users from “sample to […]
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Nanopore NO-MISS Workflow for Bacterial Genomes

We are going to use the Nanopore NO-MISS workflow. It will be used to sequence several microbial isolates. This is part of the Portable Genome Sequencing course. In preparation, this London Calling 2024 session seemed useful. Alex Trotter, Development Scientist, and Chris Alder, Bioinformatician, are both at Oxford Nanopore Technologies. They presented “How to sequence […]
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Enhanced Clinical Data Analysis with Integrated EPI2ME and MinKNOW

Tonight, I watched the London Calling 2024 session about EPI2ME workflows. The title was “Product demo: Integrated EPI2ME: streamlined analysis for clinical and biopharma applications.” Matt Parker from ONT is a clinical scientist. They spoke about AmPORE-TB, human variation workflows, and mRNA vaccine quality control. The tuberculosis workflow aims to rapidly identify antimicrobial resistance in […]
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Shifting to Oxford Nanopore for Clinical 16S rRNA Gene Sequencing

Trish Simner is the Director of Bacteriology and Infectious Disease Sequencing Laboratories. They work at the Johns Hopkins University School of Medicine in Baltimore, Maryland. They presented at London Calling 2024. The session’s title was “Bringing Nanopore Sequencing into the Clinical Microbiology Setting with Targeted Approaches.” Simner spoke about the advantages of long reads and […]
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Ultra-fast Deep-learned CNS Tumor Classification using Nanopore-sequencing

I watched a session from the Oxford Nanopore Technologies Clinical & Biopharma Day tonight. It was held on May 24, 2024 (part of London Calling 2024). Professor Pieter Wesseling is from the Princess Maxima Center for Pediatric Oncology in the Netherlands. He is also from the Amsterdam University Medical Centers. He presented the session titled […]
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