“Reveal more: bring streamlined nanopore sequencing into your lab” is the title of the Nanopore Community Meeting Houston session I watched tonight. Rachel Rubinstein, a Technical Product Manager, explained new developments to streamline nanopore sequencing. Rubinstein emphasized “end-to-end” processes. They began describing the range of the PromethION devices, from the P2 to the PromethION 48. A single PromethION flow cell can produce 50-100 Gb of >50 kb read N50 of native gDNA with the kit 14 chemistry. Simplex reads, Rubinstein explained, are useful for high-output, and duplex can aid in the assembly of complex regions. Now, duplex setup is available in MinKNOW. Duplex data can be visualized in MinKNOW and compared to simplex read Q scores. Rubinstein also shared an output graph for high-duplex flow cells accessible through MinKNOW now. The session included a video about EPI2ME updates. Real-time metagenomics, SV analyses, and other workflows can now run locally or in the cloud. Last year, Oxford Nanopore Technologies (ONT) made several updates to EPI2ME. The option to run workflows in the cloud was added. The wf-human-variation workflow was updated, and the report was enhanced. Rubinstein shared that this workflow will be added to MinKNOW. Rubinstein then shared that the TurBOT is an “end-to-end” sample prep to sequencing with methods. Onboard, there is a thermocycler and computer. The summer 2024 release will have a MinION. The release will then include a P2. Rubinstein then shared that the Mk1D MinKNOW interface has several usability updates. I was part of the usability studies and was excited to learn more about it! I hope we can use this device in the course I am developing!
