Tommaso Leonardi from the Italian Institute of Technology (IIT) presented a lightning talk at London Calling 2019 on “signal level RNA modifications detection in eukaryotic non-coding RNAs.” Leonardi explained that there are many RNA modifications, and they developed the tool Nanocompore to compare signals from two conditions. It also takes into account replicates. Nanopore also allows for multiple-factor designs and statistical analyses of batch effects. Nanocompore uses Nextflow. The workflow clusters data as modified/unmodified and the distribution in areas of interest. The tool was tested on simulated signals and then RNA modifications in human RNA. Leonardi and team also performed an m6A analysis of the human polyA transcriptome. This transcriptome analysis revealed many sites of interest that need further investigation. I wonder if Nanocompore has been used for bacterial RNA analysis?
