As part of the 2019 London Calling lightning talks, Silvia Carbonell Sala from the Centre for Genomic Regulation in Spain presented on “CapTrap-CLS: full-length RNA capture long-seq for GENCODE.” They explained how our genomes encode long non-coding RNAs. Their functions, however, may not be known. Carbonell Sala is part of the GENCODE lncRNA Capture Project to encode long non-coding RNAs.Their protocol combines capture with probes and long-read sequencing. Their system combines capture and short-read confirmation to learn about the transcriptome. However, they noticed that the transcripts they found were not always full-length. Thus, they developed a protocol for enriching for full-length sequences. Carbonell Sala concluded that Cap/Trap-CLS improved on 5’end transcript sequencing.
