The IPERT workshops finished today. I am tired and ready for rest. Tonight, I watched the London Calling 2024 session “Long-read sequencing reveals diverse patterns of epigenetic inheritance in mice.” Adam Davidovich, a Ph.D. student at Johns Hopkins, presented their work. They worked on studying intergenerational epigenetic patterns using a mouse model. The work involved characterizing allele-specific methylation and expression analysis. Davidovich designed a custom pipeline that performs methylation comparisons. Alignment and phasing are performed, creating an intermediate “pseudohybrid” genome. The meQTLs are identified as cis and trans-acting. Davidovich’s data identified genetic variants and alleles. Levels of methylation at the read level identify intermediate levels. The F2 generation is genetically diverse and distinct from the F1 parents. Davidovich concluded that long-read sequencing allowed for the analysis of genetic inheritance and methylation patterns. Admittedly, the details were complex and difficult for me to understand. I do appreciate how they used long-read sequencing and adaptive sampling to understand epigenetic inheritance patterns.
