Karen Sherwood from The University of British Columbia and Vancouver, spoke at London Calling 2025 on “Adaptive sequencing in transplantation: identifying immune-genomic risk profiles.” The Genome Canada Transplant Consortium (GCTC) was established and funded for nanopore sequencing of HLA epitopes. Now that the funding has ended the group has continued the work through another collaboration. The team did a proof-of-concept study of nanopore sequencing for precision prescribing. The test protocol can provide results within four ours. Complete sequencing of the HLA gene provides rapid information. Sherwood shared how adaptive sampling enrichment can be used to save time and perform a variety of tests. The protocol is very flexible: a BED file can be adapted for other targets.
