I finished the Nanopore Community Meeting 2022 videos and started the 2022 London Calling series. Bryant Catano is a Technical Applications Specialist with Oxford Nanopore Technologies. Catano is based at the New York offices. Their session was a masterclass on “Choosing the right nanopore sequencing device for your needs.” Catano spoke about the throughput of Flongle, MinION, and PromethION: 1.8, 43, and 245 Gbases, respectively. The Flongle flow cells with 25 kb fragments can generate 1-2 Gbases of data. Catano explained that this flow cell can be used for library quality control, plasmids, and targeted panels. The MinION with 512 pores produces 10-30 Gbases of data with an N50 of ~25 kb. The PromethION produces 100-200 Gbases with ~25 kb. The Flongle and MinION flow cells can be run on the MinION, Mk1C, and GridION. The PromethION flow cells work with the P2 devices, PromethION 24, and PromethION 48. The MinION devices produce about 50 Gbases of data, and the GridION is capable of up to 250 Gbases. The MinION Mk1C has GPU and 1 Tb SSD. The GridION Mk1 integrates high-end compute V100 GPU, 64 Gb Ram, and 4 Tb SSD. Five flow cells can be run independently or in groups. The Flongle adapter can be used too. The PromethION devices are the most powerful with highest throughput. With the P24, you can generate terabases of data. Catano explained that adaptive sampling allows enrichment or depletion of regions of interest during sequencing. While I have heard similar presentations to this one, it is helpful to learn about the output of the different devices and examples of applications.
