Metagenomics

EPI2ME Everywhere: New Workflows and Cloud Integration

We have been using EPI2ME a lot lately and want to learn more about the new features. Tonight, I watched Sirisha Hesketh, Clinical Bioinformatician from Oxford Nanopore Technologies, present “EPI2ME everywhere” at London Calling 2024. They began with an overview of the workflows available: seventeen ranging from base calling and alignment to single-cell genomics. An […]
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SamnSero Workflow: Real-time Bacterial Genome Analysis

Tonight, I watched the London Calling EPI2ME product demo: “EPI2ME: democratising bioinformatics—from point-and-click analysis to custom integrations.” Natalia Garcia, a Bioinformatics Workflow Developer with Oxford Nanopore Technologies, explained how workflows use Nextflow and Docker containers. Seventeen workflows were available at the time of the recording during London Calling. Workflows can be run via the command […]
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From Troubleshooting to Streamlining

What a weekend! I am back from the NSF ENCOUR conference. I continued watching studio interviews from London Calling 2024. Tonight, the session I watched focused on pathogen surveillance and community and collaboration. Amanda Warr from The Roslin Institute spoke about learning to use Oxford Nanopore Technologies (ONT) with metagenomic samples and troubleshooting in the […]
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The Mobile Suitcase Lab in Action

Tonight I watched the London Calling 2019 session by Ahmed Abd El Wahed from the University of Gottingen in Germany. The title of the presentation was “From ancient tomb to animal viruses: mobile suitcase lab for nanopore sequencing at field settings.” El Wahed spoke about the reagents and equipment needed to perform sequencing. They have […]
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Creating a Workflow for Pathogen Variant Detection with ONT

David Greig from Public Health England presented a session in London Calling 2019 with the title “Comparison of single nucleotide variants identified by Illumina and Oxford Nanopore technologies in the context of a potential outbreak of Shiga toxin producing.” They explained how they work on pathogen surveillance and focus on STEC: Shiga toxin-producing Escherichia coli. […]
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Fungi and Dog Skin

I started watching London Calling 2019 sessions to learn about fungal genomics and assembly. Sara D’Andreano from the Autonomous University of Barcelona in Spain gave a “lightning talk” on “MinION application: performing long-fragment analysis on pure fungal cultures (3.5 kb and 6 kb) and genome analysis of Malassezia pachydermatis.” D’Andreano is a Ph.D. student working […]
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The Applications of the Suitcase Lab

Tonight I watched a London Calling 2023 session by Ahmed Abd El Wahed from Leipzig University in Germany. The title of the session is “Pathogen and species identification using a mobile suitcase laboratory,” which aligns nicely with the course I am teaching: Portable Genome Sequencing (PGS). The recording had videos outside highlighting the suitcase lab. […]
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Sequencing in Extreme Environments Powered by the Sun

Tonight, I watched an Oxford Nanopore Technologies (ONT) video titled “Totally off-grid nanopore sequencing.” In April 2019, Glen Gowers and a group did an expedition to Vatnajokull in Iceland. They wanted to understand the invisible microbial world in extreme environments. The team had three members. The team did completely off-the-grid sequencing without backup energy. They […]
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User-friendly Computational Workflows

Tonight I watched the EPI2ME updates from Matt Parker, Associate Director of Clinical Bioinformatics at Oxford Nanopore Technologies. We will use EPI2ME next week as part of the Portable Genome Sequencing course, focusing on bacterial genome assembly and metagenomics. This Nanopore Community Meeting Houston update started with a high-energy EPI2ME video highlighting updates to compute […]
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