Reflections

Highlights from ENIGMA Workshop on Isolate Sequencing

Tonight I watched Lauren Lui from Lawrence Berkley National Laboratory present a “Long Read Isolate Sequencing and Assembly Workshop.” This session was recorded April 2, 2024 and was part of the ENIGMA program. Lui provided an overview of the apps they use: Filtlong, Unicycler, Polypolish, and Flye. The team was motivated by evolving long-read technologies […]
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Masterclass on Nanopore Sequencing: Expert Planning Tips

Tonight I watched an updated session entitled “How to get started with nanopore sequencing and plan your experiment.” The speaker was Akelia Wauchope-Odumbo, Associate Director of Technical Applications – Americas with Oxford Nanopore Technologies (ONT). Wauchope-Odumbo started this new masterclass series. The theme is “from sample to answer” covering preparation, sequencing, and analysis. The masterclass […]
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Optimizing Cell & Gene Therapies with Multi-Omic Sequencing

Heather Stark, the Global Segment Manager at Oxford Nanopore Technologies, introduced the webinar “Enhancing Cell and Gene Therapy Success with Multi-Omic Sequencing: The Oxford Nanopore Advantage.” Start covers biopharma and explained how nanopore sequencing works. They explained that there are several end-to-end workflows for synthetic biology, including plasmid, AAV, amplicon, and mRNA IVT sequencing. Clark […]
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BugSeq’s Innovative Approaches to Virus Detection

Tonight I continued watching the ONT webinar on advances in genomic profiling of respiratory viral pathogens. The second speaker was Nick Gauthier from BugSeq. The title of their session was “Translating metagenomics to the clinic.” The BugSeq team is working to develop clinical metagenomic pathogen agnostic approaches. BugSeq started working on an ONT mNGS platform […]
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Rapid Viral Genome Sequencing: Highlights from a Recent Webinar

I watched the webinar tonight facilitated by Anna Maria Niewiadomska, Global Market Segment Manager in Public Health at Oxford Nanopore Technologies. They first spoke about using ONT for microbiology and infectious disease work. The turnaround time with the rapid library prep was one feature that Niewiadomska highlighted, as well as the real-time analysis and read-length […]
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Challenges in Clinical Sequencing Techniques

Tonight, I continued watching the Oxford Nanopore Technologies London Calling 2024 expert panel on “Building a rapid, targeted, and responsive infectious disease system.”Aaron Pomerantz, the Global Segment Marketing Associate Director at ONT, facilitated the session. All panelists were invited to the stage after doing brief introductions. Rahul Batra from Guy’s and St. Thomas’ NHS Foundation Trus […]
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Building Effective Multi-Disease Surveillance Systems

Tonight, I started watching the Oxford Nanopore Technologies London Calling 2024 expert panel on “Building a rapid, targeted, and responsive infectious disease system.”Aaron Pomerantz, the Associate Director of Global Segment Marketing at ONT, helped facilitate the session. Dr. Ines Hassan from the Tony Blair Institute for Global Change started. Hassan helps encourage the development and […]
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Understanding the MinION Mk1D: Innovations for Microbiology

Tonight, I continued watching the session on the MinION Mk1D. The second speaker of the webinar was James Platt, the Director of Sequencing Platforms with Oxford Nanopore Technologies (ONT). They described the details of the MinION’s history. The Mk1D was rebuilt, including USB-C connectivity, Peltier module temperature control, and LED run status indicators. A comparison of the Mk1B […]
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Testing the MinION Mk1D in Alaskan Conditions

Tonight, I watched Devin M. Drown from the University of Alaska Fairbanks present on “Testing the MinION Mk1D in Alaska.” Drown is an Associate Professor and Faculty Director of the Institute of Arctic Biology Genomics Core. Drown is passionate about training, including doing MinION hackathons. Drown, like me, was excited to test the device. They […]
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Nanopore Sequencing: Transforming Cancer Research with Rich Data

The “Nanopore Sequencing Ultra Rich Data for Cancer Research” webinar featured Sayonika Mohanta, the Market Segment Manager for Methylation with Oxford Nanopore Technologies (ONT). Mohanta began by emphasizing that cancer research requires multimodal approaches. The example they presented was the use of WGS of cell-free or circulating tumor DNA combined with proteomics and methylation. They […]
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