sequencing

Real-time RNA Analysis with RISER

Adaptive sampling and real-time in silico enrichment have come up frequently in the last couple of months. I still haven’t used this feature and want to learn more. Tonight I watched the London Calling 2022 session by Alex Sneddon from the Australian National University entitled “RISER: real-time in silico enrichment of RNA species from nanopore […]
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Basecamp Research and Responsible Exploration

Continuing with London Calling 2022 videos, tonight I watched the session with Glen Gowers & Bupe Mwambingu from Basecamp Research. They spoke about “Responsible Exploration of our planet’s biodiversity towards discovery-enabled biodesign.” This was a showcase presentation with a few slides and and questions from the audience. Gowers is the cofounder of Basecamp Research. Mwambingu […]
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Metagenomic Analysis of Water from Brazilian Reservoirs and Ponds

I am still thinking about the Nanopore talk I watched yesterday. I now want to try using Flongle flow cells again! Tonight, I watched Douglas Mendel from the University of Sao Paulo, Brazil present at London Calling 2022. The session was only six minutes long and had the intriguing title: “Comparative metagenomics of natural and […]
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Bacterial Race Typing with the Flongle

I was excited to come across an Oxford Nanopore Technologies YouTube video from London Calling 2022 that included the use of the Flongle! Abigail Stack from Bayer Crop Sciences spoke about “The POREtal to advancing plant diagnostics: Flongle for tomato bacterial race typing and beyond.” Stack started by explaining their role as a plant pathologist […]
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ONT-based scRNAseq of Plasmablasts!

Christian Stevens from the Icahn School of Medicine at Mount Sinai presented at London Calling 2022 on “de novo assembly of immunoglobulin loci linked to full-length single-cell transcriptome of antigen-specific plasmablasts.” I was curious: what are plasmablasts? Stevens described the project as a subject who receives an MMR vaccine, at day six they extract PBMCs, […]
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An Impactful Practical

Reindert Nijland from the Wageningen University in the Netherlands wanted to teach more students about Nanopore and long-read sequencing. Nijland presented at the 2022 London Calling conference a very short (three minute) session describing how they use “Nanopore sequencing for biodiversity assessment in an educational setting.” They developed an Advanced Molecular Ecology course to help […]
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Early Detection of Changes in Esophageal Adenocarcinomas

Alvin Ng from the Early Cancer Institute at the University of Cambridge in the UK presented at London Calling 2022 on “Early detection of Barrett’s esophagus and esophageal adenocarcinoma using Oxford Nanopore long-read sequencing.” They began by explaining Barrett’s esophagus (BE) as the pre-malignant condition of esophageal adenocarcinoma (EAC) and has three stages: non-dysplastic, low-grade […]
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Ultradeep Targeted Transcript Profiling!

Rosemary Bamford from the University of Exeter, UK, presented at London Calling 2022 on “Long-read transcriptome sequencing reveals isoform diversity across human neurodevelopment and aging.” Bamford spoke about how they are using long-read transcriptome sequencing to study neuropsychiatric disorders. I didn’t know neuropsychiatric disorders have such prevalence globally! There are some findings revealing genetic risk […]
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Nanopore Sockeye

What is Nanopore Sockeye? Tonight I watched a session with Sockeye in the title and may finally learn what it does! Eoghan Harrington, a genomic applications fellow at Oxford Nanopore Technologies, presented at London Calling 2022 on “Sockeye: nanopore-only demultiplexing of single-cell reads.” They explained that there has been an explosion of single-cell applications and […]
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