Ivo Gut from the National Centre for Genomic Analysis in Spain presented at London Calling 2025 on “Going the extra mile to understand chronic lymphocytic leukaemia.” Gut explained that they wanted to focus on single-cell RNA-seq during leukemia and proposed studies to the European Research Commission. The team had preliminary data indicating two distinct clusters of cells and expression patterns. Gut explained that they focused on tonsil cells to create a “tonsil atlas” from ten donors, five data modalities, and ~357k cells. ONT provided PromethION flow cells to resequence the 10X libraries with long reads. Gut explained how they analyzed the datasets to identify the XP01 mutation. These findings may help determine mutations and problematic transcripts earlier in lymphocytic leukemia development.
