Genta Nagae from the University of Tokyo in Japan presented at the Nanopore Community Meeting in Singapore on “Direct detection of DNA modifications in human cancer genomes.” Nagae briefly spoke about the significance of DNA methylation in biology and cancer. They used the QIAamp and Puregene kits from QIAGEN to prepare tumor DNA for sequencing on a P24. Read length was 10-20 kb, and they obtained about 30X coverage. They then analyzed promoter methylation and the differential methylation of the “imprinting control region.” Long reads with Nanopore sequencing allowed for the identification of methylation patterns. Nagae and team found differentially methylated regions in mesothelioma cell lines. The research team also studied CpG island “shore” around promoters. They also detected cytosine methylation and hydroxymethylation. Nagae concluded that Nanopore sequencing provided insight into DNA modifications in human cancer genomes. I learned about different combinations of RNA sequencing and deep sequencing to learn about methylation patterns in human cell lines.
