10X Genomics

  • Home
  • Tag: 10X Genomics

Understanding Isoform Diversity in the Mouse Retina

Tonight, I watched a session on single-cell and spatial transcriptomics methods. The Oxford Nanopore Technologies (ONT) introduction highlighted how adding more cells increases resolution, yet short-read approaches have limits. Emphasizing the tagline “What’s missing matters,” the speaker noted that isoform switching is important to identify. The ONT specialist explained several solutions compatible with ONT, including […]
Read more

RAGE-seq and CITE-seq

Martin Smith from the Garvan Institute of Medical Research in Australia was mentioned in the London Calling 2019 session I watched yesterday. Today, I watched the recording of the 2019 London Calling session that Smith did on “Leveraging long reads for high-throughput multiomic analyses of cellular diversity in human tumours.” Smith wasn’t able to travel, […]
Read more

Comparison of Different scRNA-seq Methods

Mike Clark from the University of Melbourne in Australia presented at London Calling 2019 on “Deep transcriptomic sampling with long-read single cell RNA sequencing.” Clark gave the first talk in the session and explained the power of expression profiles of single cells (scRNA-seq) to identify cell types and variations in gene expression. scRNA-seq can be […]
Read more

Dark and Camouflaged Genes

Mark T.W. Ebbert from the Mayo Clinic presented at London Calling 2019 on “Long-read sequencing technologies resolve most ‘dark’ and ‘camouflaged’ gene regions.”Dark and camouflaged regions? Ebbert explained that regions can be dark because there are no reads available (“dark by depth“) or dark by low sequence quality (“dark by MAPQ“). Ebbert explained that most […]
Read more

Identifying Mechanisms of Venetoclax Resistance

Rachel Thijssen from the Walter and Eliza Hall Institute of Medical Research and Australia & Amsterdam University Medical Centers in the Netherlands presented at London Calling 2023 on “Single-cell long-read RNA sequencing reveals complex heterogeneity.” Thijssen spoke about the hallmarks of cancer and cells not responding to signals appropriately. BCL2 can be targeted in chronic […]
Read more

Ultradeep Targeted Transcript Profiling!

Rosemary Bamford from the University of Exeter, UK, presented at London Calling 2022 on “Long-read transcriptome sequencing reveals isoform diversity across human neurodevelopment and aging.” Bamford spoke about how they are using long-read transcriptome sequencing to study neuropsychiatric disorders. I didn’t know neuropsychiatric disorders have such prevalence globally! There are some findings revealing genetic risk […]
Read more

Branching Out to Sequence Single-cell Transcript Isoforms with Nanopore

Tonight I watched Sheridan Cavalier from The John Hopkins University School of Medicine present at the Nanopore Community Meeting 2022. The title of the session was “Single-cell transcript isoform sequencing of the activated adult mouse hippocampus with 10x Genomics and Oxford Nanopore.” Cavalier is a graduate student and developed an approach to sequence mouse hippocampus […]
Read more
Press ESC key to close search