enrichment

Blood Group Genome Targeted Sequencing for Precision Typing | London Calling 2024

Morgan Gueuning from the Blood Transfusion Service in Switzerland presented at London Calling 2024 on “Targeted sequencing of the entire blood group genome by adaptive sampling.” They noted that there are over 45 blood group systems coded by fifty genes and with over 350 antigens. Precise typing and matching are essential to minimize alloimmunization. Current […]
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Enriching and Guiding Sequencing

Today was Aurelio’s fifth birthday! Tonight, I watched Timothy Gilpatrick’s London Calling 2019 session on “Targeted nanopore sequencing with Cas9 for studies of methylation structural variants and mutations.” Gilpatrick is a Johns Hopkins University. They are interested in specific loci and want to generate high coverage of those areas to examine structural variation. The enrichment […]
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Metagenomics-based Infection Diagnostics

Justin O’Grady, Associate Professor in Medical Microbiology at the University of East Anglia, presented at the Nanopore Community Meeting 2017 on “Rapid metagenomic diagnosis of hospital acquired pneumonia.” They spoke about the “loss” of effective antibiotics because of rising resistance. O’Grady suggests using metagenomics-based infection diagnostics to use narrow-spectrum antibiotics. Nanopore sequencing is useful because […]
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SISPA, SASA, and Identification of Avian Influenza

Maria Chaves from Iowa State University presented at the Nanopore Community Meeting in Houston on “Enrichment strategies for recovery of Avian influenza virus from samples using MinION.” They spoke about the current highly pathogenic avian influenza (HPAI) outbreak and the importance of timely identification and characterization. Avian influenza is a huge problem for the poultry […]
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Cas9 Enrichment for ONT Sequencing

Tonight I watched the Oxford Nanopore Technologies Nanopore Learning Knowledge Exchange session on Cas9 PCR-free Enrichment. I think this kit has been discontinued, yet it is important to learn how it works. Anne-Marie Wadsworth presented the method of PCR-free enrichment using Cas9. Wadsworth noted that ONT has developed a method to use Cas9 to target […]
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Full-length Human mtDNA Sequencing with Cas9 Enrichment and ONT

Ibo Gut from CNAG-CRG in Spain presented at London Calling 2022 a session entitled “Novel method for multiplexed, full-length single-molecule sequencing of human mitochondrial DNA using Cas9-mediated enrichment.” I don’t know much about Cas9-mediated enrichment and have been curious. Gut spoke about mitochondrial DNA and how the human mitochondrial (mtDNA) is circular, 16.6 kb, has […]
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