epitranscriptome

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Detection of RNA Modifications

Eva Maria Novoa from the Center for Genomic Regulation in Spain presented at London Calling 2019 a session on “Accurate detection of m6A RNA modification in native RNA sequences using third-generation sequencing.” Novoa emphasized there is a difference between RNA levels and protein. They have been working in this field. Interestingly, RNA modifications have been […]
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Myrtle Rust and Epitranscriptomics

Ashley Jones from The Australian National University in Australia presented at the Nanopore Community Meeting in Singapore on “Exploring post-transcriptional modifications during myrtle rust pathogen-plant interactions.” Myrtle rust is a biotrophic (needs a living plant to survive) fungus Austropuccinia psidii that infects plants in the Myrtaceae family and is spreading, according to Jones. Myrtle rust […]
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Combined Transcriptome and Epitranscriptome in the Human Brain

Josie Gleeson from The University of Melbourne in Australia presented at London Calling 2023 about “Integrating the transcriptome and epitranscriptome of the human brain using direct RNA sequencing.” They spoke about the advantages of direct RNA sequencing with Oxford Nanopore Technologies: you obtain splicing patterns, isoform quantification, polyA tail length, modified nucleotides, and novel exon/intron […]
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The Epitranscriptome?

Eduardo Eyras from the Australian National University in Australia presented at London Calling 2022 a session entitled “RNA methylation detection at single-molecule resolution uncovers isoform-specific modifications during mouse brain development.” Eyras explained that chemical modifications of mRNA were discovered relatively recently and Boccaletto et al. 2021 noted >150 potential RNA modifications! However, only 6 have […]
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