Tonight, I watched the London Calling 2024 session about EPI2ME workflows. The title was “Product demo: Integrated EPI2ME: streamlined analysis for clinical and biopharma applications.” Matt Parker from ONT is a clinical scientist. They spoke about AmPORE-TB, human variation workflows, and mRNA vaccine quality control. The tuberculosis workflow aims to rapidly identify antimicrobial resistance in […]
I want to learn how to fully utilize Dorado base calling features. Tonight, I watched Susie Lee, Technical Product Manager for Basecalling and Models at Oxford Nanopore Technologies, present the latest updates to Dorado at London Calling 2024. They explained Dorado as a software tool that “powers Nanopore basecalling and more” by taking raw signal […]
Tonight I watched Seda Mirzoyan from Rutgers University present on “Using SIP and MinION sequencing to uncover active microbial communities in blueberry farm and forest soil systems.” This presentation was recorded at the NCM 2019. They spoke about characterizing active microbial communities in farms and soil systems because of the importance of blueberries in the […]
Tonight I watched the Nanopore Community Meeting Houston session by Bryant Catano, ONT Product Specialist. The session was a Masterclass entitled “How to get started with data analysis.” Catano spoke about the variety of options for data analysis available, from point-and-click to command line. Tools are available that are accessible, scalable, and versatile. Catano noted […]
The Knowledge Exchange video series had one on MinKNOW features and Updates that I watched tonight. MinKNOW has evolved in the past couple of years! Rich Roman, a Technical Product Manager from ONT, described the changes in Model Selection and incorporation of the Flip Flop Basecalling model. The high accuracy (HAC) basecalling model contains a […]
I started watching videos from the Nanopore Learning Lesson Library. The Knowledge Exchange video “Getting the most out of a flow cell” caught my attention. Connie O’Donnell was the host and was joined by Anne-Marie Wadsworth who discussed tips to maximize flow cell use. Wadsworth explained that all flow cells have a maximum run time […]
I am at ASMCUE 2023 this weekend. Students set up a sequencing run without me and it is running. Tonight I watched the Nanopore Learning video from the Human genome sequencing and analysis course “MinKNOW: Assessing your run.” Marta Verdugo from the Technical Services Team with ONT described the flow cell health and percentage of […]
Continuing with the Human genome sequencing and analysis Nanopore Learning course, I watched the session entitled “MinKNOW: Live basecalling and output folder structure.” Marta Verdugo, a member of the Technical Services Team with Oxford Nanopore Technologies, introduced the different data types and how MinKNOW processes signals into reads. Each read corresponds to a signal from […]
Dilrini De Silva, a Technical Applications Scientist in Bioinformatics at Oxford Nanopore Technologies, spoke about Methylation Detection on MinKNOW as part of the Human genome sequencing and analysis Nanopore Learning course. They discussed approaches for detecting methylation states with Nanopore sequencing and the MinKNOW software. MinKNOW includes models for methylation detection and mapping of reads. […]
Tonight I continued with the Human genome sequencing and analysis course from Nanopore Learning. I watched the video about MinKNOW Configuration for Kit 14. Bala Periaswamy from the Technical Applications Team spoke about setting up a MinKNOW run. They explained that kit 14 has improvements in the pore and motor enzyme. These changes result in […]