mtDNA

Mitochondrial Genome Sequencing Reveals Variants

“Single-cell full-length nanopore sequencing for quantitative variant analysis of native and genome-edited mitochondria” is the title of the London Calling 2023 lightning talk I watched tonight. The presenter was Mo Li from King Abdullah University of Science and Technology (KAUST) in Saudi Arabia. Li spoke about the human mitochondrial genome (mtDNA) and the challenges in […]
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Methods to Study Mitochondrial DNA Variation

Mo Li from King Abdullah University of Science and Technology (KAUST) in Saudi Arabia presented a five-minute session about “Single-cell full-length nanopore sequencing for quantitative variant analysis of native and genome-edited mitochondria.” They explained that human mitochondrial DNA (mtDNA) has a circular genome of 16.5 kb. Mutant mtDNA has an uneven distribution in tissues, and […]
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Rapid- and Ligation-based Sequencing of mtDNA

Andrea Legati from The Foundation of the Carlo BEsta Neurological Institute, IRCCS, Italy, spoke at the Nanopore Community Meeting 2021 on “Mitochondrial DNA analysis by long-read NGS in patients affected by mitochondrial diseases.” Legati spoke about the role of mitochondria in ATP production and metabolites and the significance of mitochondrial DNA (mtDNA) deletions. There are […]
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Full-length Human mtDNA Sequencing with Cas9 Enrichment and ONT

Ibo Gut from CNAG-CRG in Spain presented at London Calling 2022 a session entitled “Novel method for multiplexed, full-length single-molecule sequencing of human mitochondrial DNA using Cas9-mediated enrichment.” I don’t know much about Cas9-mediated enrichment and have been curious. Gut spoke about mitochondrial DNA and how the human mitochondrial (mtDNA) is circular, 16.6 kb, has […]
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