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Carrier Analyses using ONT

Bradley Hall from Asuragen presented at the Nanopore Community Meeting in Houston. The session was titled “AmplideX PCR and nanopore sequencing for accessible carrier screening for any lab.” Hall started by stating that “everyone is a genetic carrier of a disease or condition.” There are >2000 conditions, and carrier screening is often challenging for some […]
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Air Sampling and Metagenomic Sequencing

Christina Newman from the University of Wisconsin-Madison presented at the Nanopore Community Meeting in Houston on “Metagenomic sequencing of air samples to identify human viral pathogens.” Newman spoke about the loss of resolution of SARS-CoV-2 variants with the increased use of home kits. Newman noted that indoor air sampling could be used in schools to […]
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Candida Clinical Isolates and Reference Genome

Per Aspera Adastra from the Baylor College of Medicine and Texas Children’s Hospital, presented at the Nanopore Community Meeting in Houston on “Using long-read sequencing to generate a case-specific reference genome for pathogen strain typing.” I had watched the longer session before and will watch this one to learn more! Aspera Adastra is a clinical […]
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Fieldwork to Study Mangrome Microbial Diversity

Felipe Baez Aguirre from the University of the Andes in Colombia presented a session entitled “On-site nanopore sequencing reveals microbial diversity of a Colombian Pacific Coast mangrove soils” at the Nanopore Community Meeting in Houston. Baez Aguirre spoke about the location of the studies. They had limited access to electricity in the Bahia Malaga. The […]
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Creating Field Laboratories for Conservation Efforts

Tonight I watched a five-minute Oxford Nanopore Technologies London Calling 2022 session by Mrinalini Erkenswick Watsa and titled “Genomics in the jungle: a field laboratory success story.” Mrinalini Erkenswick Watsa is at the San Diego Zoo Wildlife Alliance and is a field scientist working on conservation. They spoke about the need to assess populations pre […]
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Ultrafast Nanopore Genome Sequencing for Critical Care

Tonight I watched the London Calling 2022 session by Euan Ashley from Stanford University. The title of this twenty-minute session was “The potential of ultrarapid nanopore genome sequencing for critical care medicine.” Ashley emphasized that the cost of genome sequencing has decreased rapidly over the last decade. With this, the accuracy has also improved. Ashley […]
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Strand-seq for Haplotyping and Parent-of-origin Assignments

Vahid Akbari is a Ph.D. student at BC Cancer in the Genome Sciences Centre in Canada. They presented at the Nanopore Community Meeting 2022 a session entitled “Simultaneous haplotyping and parent-of-origin assignment of homologous chromosomes without parental sequence data.” The approach they developed allows researchers to haplotype chromosomes and assign parent-of-origin without parent data. They […]
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The Advantages of POD5

I have been thinking and learning about POD5 files. Tonight, I watched a relevant Nanopore Community Meeting 2022 session by Alex Merry, Instrument Software Fellow at Oxford Nanopore Technologies. The session was entitled “Arrow: pointing the way forward for high-performance nanopore signal handling with POD5.” They began talking about FAST5 files to store signal data […]
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Pore-C Reveals the Chromosomal Structure of Microbial Attachment

“Use of Pore-C to explore the 3D organization of animal symbiont chromosomes” was the title of the session Tobias Viehboeck from the University of Vienna, Austria, presented at the Nanopore Community Meeting 2022. They mentioned that there are approximately 1025 microbes that live associated with plants or animals. However, Viehboeck noted that few obligate symbionts […]
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