ONT

Building Capacity and Sharing Data

Tonight I watched Zoe McDougall, VP Strategic Communications and Corporate Affairs at Oxford Nanopore Technologies (ONT), interview Rich Scott and Abdul Karim Sesay at London Calling 2023. Scott is a medic with Genomics England and was excited about using single-cell sequencing to learn about tumors. Sesay is from the MRC Unit in Gambia and spoke […]
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EPI2ME Workflows for ONT Data Analyses

Dilrini De Silva is a Field Applications Scientist in Bioinformatics for the EMEAI Region with Oxford Nanopore Technologies. De Silva presented a Masterclass at London Calling 2023 entitled “How to take your data analysis further.” This Masterclass focused on typical downstream analysis for several applications including genome assembly, variants, methylation, transcriptomics, and metagenome analysis. De […]
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Use of Oxford Nanopore Technologies for Microlab Development

Rajesh Pandey from the CSIR-Institute of Genomics and Integrative Biology in India presented at the Nanopore Community Meeting 2021 on “Genomic surveillance for future pandemic preparedness.” The lab does integrative genomics of host-pathogen interactions focusing on high-throughput and high-priority sequencing while trying to maintain cost efficiency. They have a sample to sequence microlab workflow using […]
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Near-perfect Bacterial Genomes and MAGs

Mantas Sereika, a Ph.D. student at Aalborg University in Denmark, spoke at the Nanopore Community Meeting 2021 about “Nanopore R10.4 enables near-perfect bacterial genomes.” This topic is of interest for several of the courses I teach. Sereika spoke about the improvements in accuracy of Nanopore sequencing. Raw read accuracy has increased but there are still […]
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Structural Variant Detection and Nanopore

“Comprehensive structural variant detection: from population to mosaic level” was the intriguing title for the session Fritz Sedlazeck from Baylor College of Medicine presented at London Calling 2022. Sedlazeck spoke about challenges of determining structure variants and their importance for learning about evolution, genomic disorders, their impact on regulation, and their impact on phenotypes. Sedlazeck […]
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CRISPR/Cas9 Targeted Enrichment and Whole Genome Sequencing for Personalized Medicine

Anna Dolnik from the Charite University Medicine Berlin in Germany presented at London Calling 2022 “Combined CRISPR/Cas9 targeted enrichment and whole-genome sequencing allows real-time stratification of acute myeloid leukemia (AML).” AML patient stratification, Dolnik explained, relies on identification of copy number and fusion transcripts. Dolnik mentioned they had presented on ONT-based AML systems before at […]
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Planning an Experiment with Oxford Nanopore Technologies Devices

Concetta Dipace is a Technical Services Manager with Oxford Nanopore Technologies and presented at the London Calling 2022 a masterclass introduction entitled “Getting started with nanopore sequencing and planning your experiment.” This session was similar in title to one from the Nanopore Community Meeting 2022, though there is always something new to learn in these […]
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Entering the T2T Era?

Telomere-to-telomere assembly has been coming up in Nanopore Community Meeting 2022 sessions frequently. The session I watched tonight had it in the title: “Expanding studies of global genomic diversity with complete, telomere-to-telomere assembly of diploid genomes” presented by Karen Miga from the University of California, Santa Cruz. Miga shared information about the release of the […]
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