R10.4.1

Completing Genomes of Bacterial Isolates from Outbreaks

Erin L. Young from the Department of Health and Human Services in Utah presented at the Nanopore Community Meeting in Houston on “Nanopore sequencing for enhanced antimicrobial resistance gene surveillance.” I had watched Young present previously. They spoke about using whole genome sequencing (WGS) for surveillance and its use by the Centers for Disease Control. […]
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Oxford Nanopore Technologies Kit 14 Options

The Nanopore Learning course on “Human genome sequencing and analysis” has a video on Kit V14. Vania Costa, a Technical Services Specialist with Oxford Nanopore Technologies, described the accuracy of kit 14, which relies on R10.4.1 flow cells and pores. Costa explained that kit v14 has simplex Q20+ and duplex Q30+. Currently, there are ligation-based […]
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Improved DNA and RNA Sequencing with R10.4.1 and Duplex Reads

Miten Jain from Northeastern University presented at London Calling 2023 about “A complete analysis of human genomes and transcriptomes using nanopore sequencing.” They spoke about R10.4.1 ultralong DNA sequencing. Jain’s group has been using this sample prep and flow cells with N50s between 90-110 kb and throughput of >100 Gb per PromethION flow cell. The […]
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