sequencing

March 30, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

Validation of a Rapid Glioma Molecular Detection Method

Mashiat Mimosa from the University of Toronto, Canada, spoke about “Optimization and validation of a nanopore-based sequencing method for potential molecular testing of CNS tumours.” Mimosa explained that brain tumors are the leading cause of cancer-related deaths worldwide, and glioma brain tumors account for >80% of all malignant tumors! Glioma diagnostic algorithm is very complex […]

March 24, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections, Undergraduate Research, Why

Applying Nanopore Technologies in the Undergraduate Lab

Ashley Beck from Carroll College spoke about “Applying nanopore sequencing in the undergraduate classroom for environmental microbiome analysis” at the 2022 London Calling Conference. They explained that they are dedicated to bringing technology and education to teach undergraduates. They took samples from a contaminated river from Western Montana. With students, Beck collected samples. The next […]

March 19, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

Trapping AMR Exchanges

Perfect timing! Tonight I watched Richard Goodman’s London Calling 2022 session titled “The bacterial black market: utilising sequencing to study the inter-cellular and intra-cellular transfer of AMR genes in bacteria.” I have been working on an AMR detection protocol using digital PCR for class today. Goodman is at the Liverpool School of Tropical Medicine in […]

March 18, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections, Service Learning, Softwares, Why

Creating Field Laboratories for Conservation Efforts

Tonight I watched a five-minute Oxford Nanopore Technologies London Calling 2022 session by Mrinalini Erkenswick Watsa and titled “Genomics in the jungle: a field laboratory success story.” Mrinalini Erkenswick Watsa is at the San Diego Zoo Wildlife Alliance and is a field scientist working on conservation. They spoke about the need to assess populations pre […]

March 17, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

Zooming In and Out of Multi-Omics

Dan Turner leads the Applications Team at Oxford Nanopore Technologies and presented at London Calling 2022 a session entitled “A single platform for multi-omics.” Turner mentioned that they have developed a set of posters for different applications. They spoke about epithelial to mesenchymal transition (EMT). They are able to analyze this process using Nanopore sequencing […]

March 16, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

Ultrafast Nanopore Genome Sequencing for Critical Care

Tonight I watched the London Calling 2022 session by Euan Ashley from Stanford University. The title of this twenty-minute session was “The potential of ultrarapid nanopore genome sequencing for critical care medicine.” Ashley emphasized that the cost of genome sequencing has decreased rapidly over the last decade. With this, the accuracy has also improved. Ashley […]

March 11, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

Planning an Experiment with Oxford Nanopore Technologies Devices

Concetta Dipace is a Technical Services Manager with Oxford Nanopore Technologies and presented at the London Calling 2022 a masterclass introduction entitled “Getting started with nanopore sequencing and planning your experiment.” This session was similar in title to one from the Nanopore Community Meeting 2022, though there is always something new to learn in these […]

March 10, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

Multiple Options for Nanopore Sequencing Library Prep

Tomasz Dobrzycki, a Technical Applications Scientist from Oxford Nanopore, presented at London Calling 2022 a masterclass on “Preparation: selecting the right library prep method for your experiment.” This is the second session I watch featuring Dobrzycki. This session began with the benefits of nanopore sequencing: real-time information-rich data. Oxford Nanopore Technologies (ONT) offers several options […]

March 9, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections, Softwares, Workshop, Writing

Nanopore Sequencing Devices

I finished the Nanopore Community Meeting 2022 videos and started the 2022 London Calling series. Bryant Catano is a Technical Applications Specialist with Oxford Nanopore Technologies. Catano is based at the New York offices. Their session was a masterclass on “Choosing the right nanopore sequencing device for your needs.” Catano spoke about the throughput of […]

March 8, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Reflections

NCM 2022 Update

To end the series of YouTube videos from the Nanopore Community Meeting 2022, I watched the one hour session. Rosemary started by speaking about the vision of sequencing everywhere and the technology. They spoke about supporting R9 chemistry until the end of 2023. Kit 12 was discontinued. Kit 14 will be the one used used […]