Ivo Gut from the National Centre for Genomic Analysis in Spain presented at London Calling 2025 on “Going the extra mile to understand chronic lymphocytic leukaemia.” Gut explained that they wanted to focus on single-cell RNA-seq during leukemia and proposed studies to the European Research Commission. The team had preliminary data indicating two distinct clusters […]
Ruben Cools from VIB-KU Leuven, Belgium presented at London Calling 2025 on “Bridging genotype and phenotype through long-read, single-molecule multiomics.” They are working on a high-throughput long-read single-cell open chromatin and transcriptome profiling method. The SPLOGGET approach prepares whole-genome, open chromatin and full-length transcriptome sequencing libraries for single cells. Cools explained that the method uses […]
Scott Hickey, the Director of Commercial Applications at Oxford Nanopore Technologies, spoke at the ONT Biopharma Day in Boston. The session’s title was “Antibody discovery through post-vaccination single-cell transcriptomics and haplotype-resolved germline sequencing.” Hickey spoke about a de novo full-length antibody identification through sequencing. B cell activation and proliferation were described along with the diversity […]
Tonight, I went into the lab to wash flow cells and try to reload the cDNA sequencing run. I may have caused more damaged… so I came back and watched the session “Individual cells matter – single-cell answers through nanopore sequencing.” Rachel Rubinstein, a Technical Product Manager at ONT, facilitated this showcase session. Rubinstein spoke […]
I started watching a core webinar from Oxford Nanopore Technologies (ONT) tonight. Nick Sisneros from ONT facilitated the presentation. The topic was scalable human genomic characterization with nanopore. The first presentation was by Philipp Rescheneder, Senior Director of Applications Bioinformatics at ONT. They described three end-to-end workflows using EPI2ME: the human variation, cancer genomics, and […]
Ping Lu from Peking University in China presented at London Calling 2024 on “Full-length RNA isoforms in human colorectal cancer at single-cell resolution.” Lu said colorectal cancer is the third most common cancer worldwide and the second leading cause of cancer death! Lu also mentioned that single-cell resolution is now necessary to better understand the […]
Martin Smith from the Garvan Institute of Medical Research in Australia was mentioned in the London Calling 2019 session I watched yesterday. Today, I watched the recording of the 2019 London Calling session that Smith did on “Leveraging long reads for high-throughput multiomic analyses of cellular diversity in human tumours.” Smith wasn’t able to travel, […]
Mike Clark from the University of Melbourne in Australia presented at London Calling 2019 on “Deep transcriptomic sampling with long-read single cell RNA sequencing.” Clark gave the first talk in the session and explained the power of expression profiles of single cells (scRNA-seq) to identify cell types and variations in gene expression. scRNA-seq can be […]
Colette Felton from the University of California at Santa Cruz presented at the Nanopore Community Meeting in Houston about “Haplotypes, isoforms, and fusions: towards a richer cancer transcriptome.” The Felton read studies splicing, and long reads could be used to detect isoforms. The lab developed a tool called FLAIR2 to study differential splicing. Felton’s lab […]
Tonight I watched Zoe McDougall, VP Strategic Communications and Corporate Affairs at Oxford Nanopore Technologies (ONT), interview Rich Scott and Abdul Karim Sesay at London Calling 2023. Scott is a medic with Genomics England and was excited about using single-cell sequencing to learn about tumors. Sesay is from the MRC Unit in Gambia and spoke […]