Tonight, I watched the Nanopore Learning Knowledge Exchange about Direct RNA Sequencing. Libby Snell and Botond Sipos explained how the kit and process work. Snell began by addressing the question: why use direct RNA sequencing. They explained that it is truly native RNA sequencing since the RNA strand is read directly in the Nanopore. There […]
Sam Kovaka, a PhD candidate from Johns Hopkins University, spoke at the Nanopore Community Meeting 2021 about “Visualization and analysis of nanopore RNA and DNA signal alignments with UNCALLED4.” They explained adaptive sampling (AKA “ReadUntil“): “software-based targeted sequencing via selective ejection of reads” Kovaka explained that this requires rapid identification based on the beginning of […]



