Month: September 2024

Latest EPI2ME Features for Variant Analysis Explained

Matt Parker, the Director of Clinical Bioinformatics Software at Oxford Nanopore Technologies, facilitated the Nanopore Community Meeting Boston 2024 session on variant analysis with EPI2ME. The title of the presentation was “Ultra-rich human data – variant analysis with EPI2ME.” They shared the EPI2ME user interface updates that were recently released. There is a new element […]
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Revolutionizing DNA Methylation Detection with Nanopore Sequencing

Dmitrijs Rots from Erasmus MC in the Netherlands presented at the Nanopore Community Meeting in Boston (NCM Boston 2024) on “DNA methylation signature detection using ultra-rapid, long-read nanopore genome sequencing.” Rots mentioned that Erasmus MC is one of the largest hospitals in Europe! The team wanted to implement nanopore sequencing to test for methylation and […]
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Exploring Single-Cell Sequencing with Oxford Nanopore

Tonight, I went into the lab to wash flow cells and try to reload the cDNA sequencing run. I may have caused more damaged… so I came back and watched the session “Individual cells matter – single-cell answers through nanopore sequencing.” Rachel Rubinstein, a Technical Product Manager at ONT, facilitated this showcase session. Rubinstein spoke […]
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ElysION Nanopore Sequencing: Streamlined DNA Extraction

Jade Bartolo, the Associate Director for Automation at Oxford Nanopore Technology (ONT), introduced the ElysION. The five-minute recording is titled “Smarter genomics for streamlined answers – ElysION, sample-to-answer nanopore sequencing.” The ElysION has an integrated thermocycler and a MinION Mk1D or PromethION P2. The system can also shake and is compatible with tubes and plates. […]
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Understanding the NO-MISS Workflow for Microbial Sequencing

Tonight, I watched the second half of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” Different extraction methods produced varying yields. Bead-beating and enzymatic lysis extractions affect read length and throughput, while fungal samples produce lower yields. Some potential issues include incomplete lysis and contaminants. Additional clean-up […]
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DNA Extraction Techniques: Bead Beating vs Enzymatic Lysis

We hosted a bioinformatics workshop with Oxford Nanopore Technologies specialists! It was fun to learn about EPI2ME updates. Tonight, I watched the beginning of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” The webinar began with a review of the applications related to microbial isolate […]
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Advancements in Nanopore Sequencing for Microbial Genomics

I continued watching the “Enhanced microbial profiling and metagenomics with nanopore sequencing” webinar. The second speaker was Steven Batinovic, a Field Application Scientist with Oxford Nanopore Technologies (ONT). The title of their session was “What you’re missing matters: delivering the future of microbial genomics with Oxford Nanopore.” Batinovic started by listing the microbiology and infectious […]
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Comparing Nanopore and PacBio: Microbial Community Profiling

Tonight, I watched a Nanopore webinar by Tianyuan Zhang. The session’s title is “The Newest Oxford Nanopore R10.4.1 Full-length 16S rRNA Sequencing Enables the Accurate Resolution of Species-Level Microbial Community Profiling.” Zhang spoke about the resolution gains with full-length 16S sequencing, citing a Nature article from 2019. However, the error rate of ONT was previously […]
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Guide to Loading PromethION Flow Cells

I watched an updated version of the “Hot to load a PromethION Flow Cell” training tonight. Tomek Dobrzycki, a Field Applications Scientist from Oxford Nanopore Technologies, was the presenter. This is part of the masterclass series with the tagline “from sample to answer.” Dobrzycki began by explaining how one nanopore per well can be arranged. These […]
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Advancements in Direct RNA Nanopore Sequencing

Tonight, I continued watching a Knowledge Exchange about direct RNA sequencing. Daniel Garalde, Associate Director of Technology Markets with Oxford Nanopore Technologies, introduced the Knowledge Exchange webinar I watched tonight. The title of the Knowledge Exchange is “How direct RNA nanopore sequencing can enhance your research.” Libby Snell, Director of RNA and cDNA Sample Technology, presented an update […]
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