Hazel Johnson, member of the Technical Services Team at Oxford Nanopore Technologies, presented the second video in the Nanopore Learning Human Genome Sequencing and Analysis course. They shared the range of products for sample preparation, instruments, and software offered by ONT. Johnson shared a graphic with the nanopore sequencing platforms. The Flongle is low cost and can produce 1-3 Gbases of data. The MinION can be plugged into a laptop and produce up to 50 Gbases of data. The MinION MK1C offers portability with powerful portability with GPU and screen. The Flongle is a single-use flow cell for smaller tests and experiments. It is compatible with the MinION and GridION. You do need a Flongle adapter to dock with a MinION or GridION. The GridION has five positions and has been designed to run and analyze the positions together or independently. The GridION can run MinION and Flongle flow cells. Next, the PromethION offers up to 24 or 48 high-capacity flow cells. The system has a compute unit and a sequencing unit. Multiple flow cells can be assigned to an experiment. This video was a quick overview of the different systems offered by ONT. It did not mention the P2… but the next session did! Kieran Hejmadi from the Technical Product group from ONT provided a tour of the P2 Solo. They noted that only the newer GridIONs are compatible with the P2 Solo. When connected and powered, the P2 has a green light on the bottom right of the grill. Once ready, the LEDs on top turn blue/solid with the hardware check flow cells (“CTCs”). After completing the hardware check, the LEDs change from a green scrolling pattern to a solid green pattern. The video was helpful to learn how to set up the P2 solo on a laptop and what the lights represent (during a hardware check).
