Metagenomics

Assembling Redwoods, Giant Sequoia, and a Fungus

“Sequencing and assembling the mega-genomes of mega-trees: the giant sequoia and coast redwood genomes” is the title of the session Steven Salzberg from Johns Hopkins University presented. I was curious why this session was classified as “Metagenomics.” Salzberg works on several different organisms. The project’s first results were released in April 2019, describing the genome […]
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Metagenomics-based Infection Diagnostics

Justin O’Grady, Associate Professor in Medical Microbiology at the University of East Anglia, presented at the Nanopore Community Meeting 2017 on “Rapid metagenomic diagnosis of hospital acquired pneumonia.” They spoke about the “loss” of effective antibiotics because of rising resistance. O’Grady suggests using metagenomics-based infection diagnostics to use narrow-spectrum antibiotics. Nanopore sequencing is useful because […]
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MinHash for Rapid Pathogen Identification

Brian Ondov from the National Biodefense Analysis and Countermeasures Centre (NBACC) spoke at the Nanopore Community Meeting 2015 on “Leveraging MinHash for rapid identification of nanopore data on mobile hardware.” Ondov explained how the portability of the MinION device can be paired with portable hardware. They noted that memory expansion on a small device can […]
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Rich Activated Sludge Communities

Caitlin Singleton from Aalborg University in Denmark presented at the Nanopore Community Meeting 2020 a session on metagenomic analyses titled “From MAGs to riches: exploring the microbial community of activated sludge using 1,083 high-quality metagenome-assembled genomes.” Singleton is a postdoc and explained why activated sludge is important. Activated sludge is used to treat wastewater to […]
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Nanopore Sequencing and Bacterial Genome Assembly

Mantas Sereika from Aalborg University in Germany presented at the Nanopore Community Meeting 2021 a session with a title that caught my attention tonight: “Nanopore R10.4 enables near-perfect bacterial genomes.” They spoke about Nanopore sequencing raw read accuracy improvements and issues with homopolymers. Insertions and deletions in homopolymer regions can be an issue causing frameshifts. […]
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Air Sampling and Metagenomic Sequencing

Christina Newman from the University of Wisconsin-Madison presented at the Nanopore Community Meeting in Houston on “Metagenomic sequencing of air samples to identify human viral pathogens.” Newman spoke about the loss of resolution of SARS-CoV-2 variants with the increased use of home kits. Newman noted that indoor air sampling could be used in schools to […]
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Completing Genomes of Bacterial Isolates from Outbreaks

Erin L. Young from the Department of Health and Human Services in Utah presented at the Nanopore Community Meeting in Houston on “Nanopore sequencing for enhanced antimicrobial resistance gene surveillance.” I had watched Young present previously. They spoke about using whole genome sequencing (WGS) for surveillance and its use by the Centers for Disease Control. […]
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Fieldwork to Study Mangrome Microbial Diversity

Felipe Baez Aguirre from the University of the Andes in Colombia presented a session entitled “On-site nanopore sequencing reveals microbial diversity of a Colombian Pacific Coast mangrove soils” at the Nanopore Community Meeting in Houston. Baez Aguirre spoke about the location of the studies. They had limited access to electricity in the Bahia Malaga. The […]
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Real-time Analysis with EPI2ME

Tonight I watched another video from the Nanopore Learning course on Human Genome Sequencing and Analysis. This session was an introduction to real-time analysis. Sandra Guerra, a member of the Technical Services Specialist, introduced the tools for real-time data analysis. Guerra described the process of Nanopore DNA/RNA sequencing. They noted that Nanopore technologies and devices […]
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