“Nanopore sequencing of wild virus particles reveals previously undetected phage and phage-parasitizing elements” was the title of the London Calling 2023 session Ed DeLong from the University of Hawaii at Manoa did. They began with an explanation of how phages affect algae and carbon cycles in the ocean. Some viruses can cause aggregation and different […]
Fuchou Tang from the Biomedical Pioneering Innovation Center in Peking University, China, spoke at London Calling 2023 about “scNanoATAC-seq: a long-read single-cell assay to simultaneously detect chromatin accessibility and genetic variants.” Tang spoke about developing single-cell genomics tools as a postdoctoral researcher. Then, in 2013, they developed individual cell methylation tools. Tang and team continued […]
Mantas Sereika from Aalborg University in Denmark presented at the nanopore Community Meeting 2022 on “Targeted deep metagenomics for the recovery of novel closed microbial genomes from highly complex communities.” I thought this ten-minute session had an intriguing title, as I begin to wonder what we will do next in the BIT 295 course in […]
The Dorado basecaller has been on my mind and appearing in different Nanopore resources. Tonight I watched Mike Vella, Director of Machine Learning Operations at Oxford Nanopore Technologies, present an update on Dorado at the Nanopore Community Meeting 2022. Vella spoke about why we need another basecaller, as Nanopore has Bonito and Guppy. Vella explained […]
Tonight I watched two short Nanopore Community Meeting 2022 recordings. I started with Sarah Scott from the the Minnesota Public Health Laboratory, USA who presented on the “Exploration of Oxford Nanopore long-read sequencing to contribute to influenza surveillance at the state level.” Scott is an APHL and CDC Fellow. Scott explained that genomic surveillance of […]
Kathleen Zeglinski from the Walter and Eliza Hall Institute of Medical Research in Australia presented the ten minute recorded session entitled “NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cells” at the Nanopore Community Meeting 2022. Zeglinski explained that hybridomas and B cells are common […]
Katherine Lawrence, a Machine Learning Bioinformatician with Oxford Nanopore, spoke at the Nanopore Community Meeting 222 about “Advances in duplex basecalling.” They defined duplex sequencing as both ends of the DNA with adaptor motor proteins and two reads from the same molecule. Duplex basecalling incorporates information from both reads’ signals and improves accuracy with the […]
Gabriela Pozo spoke at the Nanopore Community Meeting 2022 about “Whole-genome sequencing of the Ecuadorian brown-headed spider monkey (Ateles fusciceps fusciceps), a critically endangered species. Pozo is from the Universidad San Francisco de Quito, Ecuador. Pozo mentioned that this is a diurnal primate, with a mostly ripe fruit diet and also mushrooms and insects, and […]
Marcus Stoiber, a Principle Algorithms Researcher with Oxford Nanopore Technologies, presented at the Nanopore Community Meeting 2022 the “Nanopore modified base update.” Stoiber explained Oxford Nanopore sequencing allows for powerful epigenetics research because it can “directly detect methylation with high reproducibility and low bias.” You can sequence native DNA and RNA to assess modification. The […]
Sarah Frampton from the University of Southampton, UK spoke at the Nanopore Community Meeting 2022 on “Shinning a light on the FCGRs: deconvoluting their challenging (epi)genomic organisation and consequences in immune oncology research.” Frampton is a Ph.D. student and spoke about monoclonal antibodies and their therapeutic potential. The Fc gamma receptors, Frampton explained, are important […]