Reflections

Targeted Nanopore Sequencing to Detect Immune Escape Variants

The five minute London Calling 2023 session entitled “Targeted nanopore sequencing using hybridisation probes reveals immune escape polymorphisms in malaria vaccine candidates” was a talk by Alyssa Barry from Deakin University in Australia. They spoke about the global number of cases of malaria, deaths, and people at risk. Malaria vaccine is challenging because of the […]
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Rapid Biodiversity Assessments thorough mtDNA Sequencing

Mariana Corrales Orozco from EAFIT University in Colombia spoke at London Calling 2023 about “Revolutionizing biodiversity research: Oxford Nanopore sequencing for the rapid and accurate identification of endangered species.” They started with heat maps depicting species diversity and the challenges of identifying new species. Typically, an expert taxonomist examines a sample. This approach requires sacrificing […]
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EPI2ME Labs Metagenomics Workflows on the Cloud

Tonight I watched the London Calling 2023 product demo for “Analysing bacterial genomes – from species identification to AMR.” Stephen Rudd, Director of Bioinformatics Product at Oxford Nanopore Technologies (ONT), shared their work on EPI2ME labs products. They have been developing EPI2ME Labs workflows for several years. These are pre-installed workflows that can help solve […]
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Workflows for Metagenomics and Isolate Genomes with ONT

The London Calling Metagenomics and isolate genome sequencing product demo was a fourteen-minute recording I watched tonight. The session was hosted by Rachel Rubinstein, a Technical Product Manager, Software, for Oxford Nanopore Technologies (ONT). Rubinstein spoke about using ONT for bacterial and viral genome sequencing. The goal of the session was to share applications using […]
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Developments in ONT from LC2023

Tonight I watched the “Welcome back to London Calling 2023” session. Rosemary Sinclair Dokos, Senior VP of Product & Programme Management at Oxford Nanopore Technologies (ONT) was the presenter and began day two of London Calling 2023 by talking about the journey of ONT. Sinclair Dokos found several parallels between the London Underground construction and […]
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EPI2ME Workflows for ONT Data Analyses

Dilrini De Silva is a Field Applications Scientist in Bioinformatics for the EMEAI Region with Oxford Nanopore Technologies. De Silva presented a Masterclass at London Calling 2023 entitled “How to take your data analysis further.” This Masterclass focused on typical downstream analysis for several applications including genome assembly, variants, methylation, transcriptomics, and metagenome analysis. De […]
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Which ONT library prep kit do I choose?

Akelia Wauchope-Odumbo, a Technical Services Manager – Americas with Oxford Nanopore Technologies (ONT), led a Masterclass at London Calling 2023. I watched the recording tonight, as the session title aligns with the course goals for the ONT-based lab I am designing: “How to get started with nanopore sequencing and plan your experiment.” The session started […]
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Nanopore Sequence Analysis and Methylation Sequencing

Bryant Catano, a Product Support Scientist with Oxford Nanopore Technologies, led a Masterclass at London Calling 2023 entitled “How to get started with data analysis.” I have watched several of the other courses. This session focused on base calling, file formats, and MinKNOW and EPI2ME. Catano also discussed methylation tools. Catano started by emphasizing that […]
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