sequencing

Gbases and Basecallig

Continuing with the Human genome sequencing and analysis Nanopore Learning course, I watched the session entitled “MinKNOW: Live basecalling and output folder structure.” Marta Verdugo, a member of the Technical Services Team with Oxford Nanopore Technologies, introduced the different data types and how MinKNOW processes signals into reads. Each read corresponds to a signal from […]
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Identifying Methylation States with MinKNOW

Dilrini De Silva, a Technical Applications Scientist in Bioinformatics at Oxford Nanopore Technologies, spoke about Methylation Detection on MinKNOW as part of the Human genome sequencing and analysis Nanopore Learning course. They discussed approaches for detecting methylation states with Nanopore sequencing and the MinKNOW software. MinKNOW includes models for methylation detection and mapping of reads. […]
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Handling ONT Flowcells

Every time I load another Oxford Nanopore Technologies (ONT) flow cell, I learn something new. Tonight, I watched the Nanopore Learning course on “Human genome sequencing and analysis” and the video about flow cell handling. Hazel Johnson, a Technical Services Specialist with ONT, explained the three flow cells available for ONT. The Flongle is designed […]
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The MinKNOW Graphical User Interface (GUI)

Tonight I watched the MinKNOW GUI video from the Nanopore Learning course on Human Genome Sequencing and Analysis. Before watching these videos, I reviewed the Kit 14 videos. The MinKNOW graphical user interface (GUI) session was presented by Marta Verdugo from the Technical Services Team at Oxford Nanopore Technologies (ONT). The MinKNOW software is used […]
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Oxford Nanopore Technologies Kit 14 Options

The Nanopore Learning course on “Human genome sequencing and analysis” has a video on Kit V14. Vania Costa, a Technical Services Specialist with Oxford Nanopore Technologies, described the accuracy of kit 14, which relies on R10.4.1 flow cells and pores. Costa explained that kit v14 has simplex Q20+ and duplex Q30+. Currently, there are ligation-based […]
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The Versatile LSK

Continuing with the Nanopore Learning course on Human genome sequencing and analysis, tonight I watched the video with the title “Library preparation: Ligation sequencing kit.” Hazel Johnson, a member of the Technical Services Team with Oxford Nanopore Technologies, explained the basics of library preparation for Nanopore sequencing. They defined library preparation as “converting sample into […]
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