sequencing

Suggested Approaches for High-quality DNA and RNA

Vania Costa, a Field Applications Scientist with Oxford Nanopore Technologies (ONT), led the Masterclass I watched tonight. This session was part of London Calling 2023 and entitled “How to extract high-quality DNA and RNA.” I have watched a couple of Masterclasses Costa has created. The objective of this session was for users to determine appropriate […]
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Which ONT library prep kit do I choose?

Akelia Wauchope-Odumbo, a Technical Services Manager – Americas with Oxford Nanopore Technologies (ONT), led a Masterclass at London Calling 2023. I watched the recording tonight, as the session title aligns with the course goals for the ONT-based lab I am designing: “How to get started with nanopore sequencing and plan your experiment.” The session started […]
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Nanopore Sequence Analysis and Methylation Sequencing

Bryant Catano, a Product Support Scientist with Oxford Nanopore Technologies, led a Masterclass at London Calling 2023 entitled “How to get started with data analysis.” I have watched several of the other courses. This session focused on base calling, file formats, and MinKNOW and EPI2ME. Catano also discussed methylation tools. Catano started by emphasizing that […]
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Sequencing Borg Genomes

Marie Charlotte Schoelmerich from the University of California, Berkley presented at London Calling 2023 about “Confirmation of borg extrachromosomal genomes: analysis of their methylation patterns, metabolism, and tandem repeats.” Schoelmerich is a postdoc in Jill Banfield’s lab and spoke about the key role of microorganisms in methane cycles. Methane emissions are driving climate change, and […]
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Swab-to-Sequencer in Space

Sarah Stahl-Rommel from NASA Health and Human Performance Contact & JES Tech in the USA presented at London Calling 2023 a session entitled “Unlocking the microbiome of the International Space Station.” Stahl-Rommel explained that NASA has been using culture-based approaches for decades. These assessments require samples transported back to earth. Now, they are preparing to […]
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Surveillance Efforts in New Zealand

Joep de Ligt from the Institute of Environmental Science and Research Limited (ESR) in New Zealand presented at London Calling 2023 a session entitled “The value of real-time, long-read sequencing for public health.” They talked about how they developed technologies during the COVID pandemic. The ESR uses genomics for public health. de Ligt described how […]
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Rapid Tuberculosis Drug Resistance Profiling from Sputum

Tonight the London Calling 2023 session I watched was presented by Tiana Schwab from the Institute of Social and Preventative Medicine at the University of Bern in Switzerland. The title is “Tuberculosis drug resistance profiling from native sputum using nanopore targeted sequencing: a field application study.” Schwab is working on a Ph.D. and described the […]
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nCATS to Sequence Mobile Element Insertions

Camille Mumm, a graduate student at the University of Michigan, presented at London Calling 2023 a five-minute session entitled “Exploring the impact of mobile elements on Alzheimer’s disease using targeted long-read sequencing. They explained that Alzheimer’s disease (AD) is a devastating neurodegenerative disease with not yet fully characterized genetic mechanisms. Somatic mutations in the brains […]
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