Pore-C is a method I hear about, yet I don’t fully understand the details. Eoghan Harrington from Oxford Nanopore Technologies (ONT) presented at London Calling 2019 a session titled “Pore-C: a method for genome-wide, multi-contact chromosome conformation capture.”Harrington is part of the applications team and focuses on genomic applications. They collaborate with various partners. The 3D Genome and spatial structure of chromatin within a cell can be studied with chromatin capture. The techniques create chromatin contact maps. Regions that are in close contact are close together on the linear map. Harrington noted that two samples can be compared in a graph. The methods have evolved from 3C, 4C, 5C, Hi-C, and MC4C! The protocol for Pore-C starts by crosslinking the DNA within the nucleus. The protocol is optimized to allow for simple sample prep and high-resolution maps. Harrington and the team are trying several improvements, including simple selection. Copy number and structural variation metrics can be enabled. One day, I would like to try this technique.
