16S

PCR-based ONT Kits and 16S Analysis

Tonight I continued watching the Nanopore Learning Metagenomics series. The lessons focused on Library Preparation: Low input PCR kits. Hazel Johnson spoke about low-input kits. Library preparation involves attachment of sequencing adapters that are “Y” shaped and include a motor protein and leader sequence. A tether site facilitates the capture of the molecule. When you […]
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Adaptive Sampling for In-depth Amniotic Infection Analyses

One of the London Calling 2023 Showcase Stage sessions was on Targeted Sequencing. Tonight, i watched the four-minute recording entitled “Rapid identification of bacterial invasion: full-length 16S or adaptive sampling?” The title intrigued me! I am waiting for the release of the updated 16S amplicon kit. There were several panelists and Thidathip Wongsurawat from the […]
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Undergraduate Research with the CREWS Project

Megan Radosevich from Carroll College presented at the Nanopore Community Meeting 2021 on “Analyzing regional and seasonal microbial community variation from a contaminated Montanana river.” Their project is called CREWS: Consortium for Research on Environmental Water Systems that is funded by the Montana NSF EPSCoR Partnership with several colleges and universities in Montana. A central […]
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