DNA

Understanding the NO-MISS Workflow for Microbial Sequencing

Tonight, I watched the second half of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” Different extraction methods produced varying yields. Bead-beating and enzymatic lysis extractions affect read length and throughput, while fungal samples produce lower yields. Some potential issues include incomplete lysis and contaminants. Additional clean-up […]
Read more

DNA Extraction Techniques: Bead Beating vs Enzymatic Lysis

We hosted a bioinformatics workshop with Oxford Nanopore Technologies specialists! It was fun to learn about EPI2ME updates. Tonight, I watched the beginning of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” The webinar began with a review of the applications related to microbial isolate […]
Read more

Advancements in Nanopore Sequencing for Microbial Genomics

I continued watching the “Enhanced microbial profiling and metagenomics with nanopore sequencing” webinar. The second speaker was Steven Batinovic, a Field Application Scientist with Oxford Nanopore Technologies (ONT). The title of their session was “What you’re missing matters: delivering the future of microbial genomics with Oxford Nanopore.” Batinovic started by listing the microbiology and infectious […]
Read more

Advancements in Direct RNA Nanopore Sequencing

Tonight, I continued watching a Knowledge Exchange about direct RNA sequencing. Daniel Garalde, Associate Director of Technology Markets with Oxford Nanopore Technologies, introduced the Knowledge Exchange webinar I watched tonight. The title of the Knowledge Exchange is “How direct RNA nanopore sequencing can enhance your research.” Libby Snell, Director of RNA and cDNA Sample Technology, presented an update […]
Read more

Understanding Human Variation and Cancer Genomics via EPI2ME

I started watching a core webinar from Oxford Nanopore Technologies (ONT) tonight. Nick Sisneros from ONT facilitated the presentation. The topic was scalable human genomic characterization with nanopore. The first presentation was by Philipp Rescheneder, Senior Director of Applications Bioinformatics at ONT. They described three end-to-end workflows using EPI2ME: the human variation, cancer genomics, and […]
Read more

Evolution of MinION Technology in Microbiology: ASM Microbe 2024 Session Insights by Joshua Quick

Today, I watched the beginning of an Oxford Nanopore Technologies special recording. The title is “Celebrating a decade of DNA discoveries: 10 years of the MinION in microbiology.” This session was from June 14th, 2024, at the ASM Microbe meeting. Pomeranz began by introducing the ONT and noting that there have been over 13,000 publications […]
Read more

Real-time Genomic Characterization of Pediatric Acute Leukemia: UNC Research

The second talk in the session I started watching yesterday was by a postdoctoral fellow from UNC. Julie Geyer from the University of North Carolina Chapel Hill School of Medicine described their research. The title of the talk was “Real-time genomic characterization of pediatric acute leukemia using adaptive sampling.” Leukemia is cancer of the blood. […]
Read more

Long-read Sequencing and Structural Variants in Precision Oncology

“Accelerating precision oncology research with nanopore sequencing” is the name of the session I watched tonight. Anna Dysko, Associate Director of Business Development with Oxford Nanopore Technologies, facilitated the discussion on April 8, 2024. Dysko explained how nanopore sequencing works: DNA or RNA is passed through a nanopore thanks to the action of a motor […]
Read more

Genomics England and ONT

I continued watching a longer session from Oxford Nanopore Technologies (ONT) summer programming tonight. “The era of complete genomes, at any scale” was the title of a session Cora Vaher gave. They talked about how methylation can be detected without additional library preparation. Haplotype-specific methylation could be very useful in learning about genetic diseases. Vaher shared a […]
Read more

Unlocking Genomic Frontiers: ONT’s Long-Read Sequencing Breakthroughs

Tonight I started watching a longer session from Oxford Nanopore Technologies (ONT) summer programming. “The era of complete genomes, at any scale” was the title of a session Cora Vaher gave. They described how long-read sequencing with ONT has changed sequencing. Vaher stated that “the era of full genomes is coming” because there are many […]
Read more
Press ESC key to close search