genetics

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Mapping Active rRNA Gene Clusters in Arabidopsis

Tonight, I watched a shorter Nanopore Community Meeting Boston session. Anastasia McKinlay from Indiana University & HHMI presented on “Positional mapping of active versus silenced rRNA gene clusters within A. thaliana nucleolus organizer regions. McKinlay spoke about nucleolus organizer regions in Arabidopsis thaliana. These positions are difficult to understand with shorter reads. Long-read sequencing helped […]
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Revolutionizing DNA Methylation Detection with Nanopore Sequencing

Dmitrijs Rots from Erasmus MC in the Netherlands presented at the Nanopore Community Meeting in Boston (NCM Boston 2024) on “DNA methylation signature detection using ultra-rapid, long-read nanopore genome sequencing.” Rots mentioned that Erasmus MC is one of the largest hospitals in Europe! The team wanted to implement nanopore sequencing to test for methylation and […]
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Understanding the NO-MISS Workflow for Microbial Sequencing

Tonight, I watched the second half of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” Different extraction methods produced varying yields. Bead-beating and enzymatic lysis extractions affect read length and throughput, while fungal samples produce lower yields. Some potential issues include incomplete lysis and contaminants. Additional clean-up […]
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DNA Extraction Techniques: Bead Beating vs Enzymatic Lysis

We hosted a bioinformatics workshop with Oxford Nanopore Technologies specialists! It was fun to learn about EPI2ME updates. Tonight, I watched the beginning of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” The webinar began with a review of the applications related to microbial isolate […]
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Advancements in Nanopore Sequencing for Microbial Genomics

I continued watching the “Enhanced microbial profiling and metagenomics with nanopore sequencing” webinar. The second speaker was Steven Batinovic, a Field Application Scientist with Oxford Nanopore Technologies (ONT). The title of their session was “What you’re missing matters: delivering the future of microbial genomics with Oxford Nanopore.” Batinovic started by listing the microbiology and infectious […]
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Advancements in Direct RNA Nanopore Sequencing

Tonight, I continued watching a Knowledge Exchange about direct RNA sequencing. Daniel Garalde, Associate Director of Technology Markets with Oxford Nanopore Technologies, introduced the Knowledge Exchange webinar I watched tonight. The title of the Knowledge Exchange is “How direct RNA nanopore sequencing can enhance your research.” Libby Snell, Director of RNA and cDNA Sample Technology, presented an update […]
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Understanding Human Variation and Cancer Genomics via EPI2ME

I started watching a core webinar from Oxford Nanopore Technologies (ONT) tonight. Nick Sisneros from ONT facilitated the presentation. The topic was scalable human genomic characterization with nanopore. The first presentation was by Philipp Rescheneder, Senior Director of Applications Bioinformatics at ONT. They described three end-to-end workflows using EPI2ME: the human variation, cancer genomics, and […]
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Tonight, I continued watching the Oxford Nanopore Technologies session from ASM Microbe 2024. The title of the session was “Celebrating a decade of DNA discoveries: 10 years of the MinION in microbiology.”Before the second speaker, the live sequencing demo was shown on the screen. The team was sequencing a microbe and had the audience guess […]
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Evolution of MinION Technology in Microbiology: ASM Microbe 2024 Session Insights by Joshua Quick

Today, I watched the beginning of an Oxford Nanopore Technologies special recording. The title is “Celebrating a decade of DNA discoveries: 10 years of the MinION in microbiology.” This session was from June 14th, 2024, at the ASM Microbe meeting. Pomeranz began by introducing the ONT and noting that there have been over 13,000 publications […]
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Real-time Genomic Characterization of Pediatric Acute Leukemia: UNC Research

The second talk in the session I started watching yesterday was by a postdoctoral fellow from UNC. Julie Geyer from the University of North Carolina Chapel Hill School of Medicine described their research. The title of the talk was “Real-time genomic characterization of pediatric acute leukemia using adaptive sampling.” Leukemia is cancer of the blood. […]
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