Nanopore

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Nanopore Sequencing in Cell Therapy Testing

Stacy L. Springs from the MIT Center for Biomedical Innovation presented at the NCM Biopharma Day in Boston on “Application of nanopore long-read sequencing to sterility testing for cell therapy products.” Springs spoke about the diversity of impurities in biological medicines. They spoke about the need for rapid sterility testing in cell therapy manufacturing. Springs […]
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Comparing Nanopore and PacBio: Microbial Community Profiling

Tonight, I watched a Nanopore webinar by Tianyuan Zhang. The session’s title is “The Newest Oxford Nanopore R10.4.1 Full-length 16S rRNA Sequencing Enables the Accurate Resolution of Species-Level Microbial Community Profiling.” Zhang spoke about the resolution gains with full-length 16S sequencing, citing a Nature article from 2019. However, the error rate of ONT was previously […]
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Empowering Global Surveillance: CDC’s Training Success

I continued watching the “Celebrating a decade of DNA discoveries: 10 years of the MinION in microbiology” ASM Microbe 2024 session. Matthew Keller, a Biologist with the Genomics & Diagnostics division at the Centers for Disease Control, was the last speaker. Keller’s team focuses on sequencing influenza viruses to monitor seasonal dynamics/surveillance. However, Keller noted […]
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Nanopore Sequencing Library Prep: Techniques and Comparison

Tonight, I watched Jessica Anderson, a Senior Field Applications Scientist with Oxford Nanopore Technologies (ONT), present at London Calling 2024. The session was part of the Masterclass 2024 series. It was titled “How to select the right library prep workflow for your experiment.” This session started with an introduction describing how nanopore sensing works. Anderson explained that during […]
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Genomic Surveillance of Multi-Drug Resistant Organisms with Long-Read Sequencing

Today was the first day of our IPERT Summer Workshops! I also started watching London Calling 2024 videos available on the YouTube channel. I began with the session titled “Genomic surveillance of multidrug-resistant organisms based on long-read sequencing.” Fabian Landman works at the Centre for Infectious Disease Control in The Netherlands and the Dutch National […]
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Oxford Nanopore Technologies: Advancing Research and Applications in the Next Decade

Tonight, I watched another London Calling 2024 studio interview. Zoe McDougall previously interviewed two of the three guests. Ronja Friedhoff is a master’s student at the Technische Universitat Braunschweig in Germany. They explained the benefits of networking opportunities at London Calling 2024: Friedhoff learned about new research areas and met new people. Ivo Gut from […]
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From Plant to Publication in a Couse

I have been curious about the title of a session from London Calling 2024: “Empowering scientists with data literacy skills in long-read genomic sequencing.” Katharina Wolff from TU Braunschweig in Germany was the presenter and explained the wide variety of plants and potential for genome sequencing. Wolf and the team wanted to enable students to […]
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The Challenges of Sequencing tRNAs

Tonight, I watched the London Calling 2019 session by Irina Chelysheva from the University of Hamburg in Germany. The session’s title is “Small, modified, and highly structured: the challenge of tRNA sequencing.” Their lab is interested in sequencing tRNA because it is challenging. The major problems for sequencing, Chelysheva noted, include the short length of […]
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Forensic Profiling with Nanopore Sequencing

Rebecca Richards from the University of Auckland in New Zealand presented at London Calling 2019 on “Biological evidence of the future: the use of sequencing in forensic science.” They spoke about forensic DNA profiling by analyzing STRs and capillary gel electrophoresis. This analysis looks at the length of the repeats. However, there are limitations: no […]
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