ONT

Microbial Identification Methods in Biopharma

Radhakrishna Tirumalai from Meck presented at the ONT Biopharma Day in Boston. The session, “Microbial identification in biopharma, ” focused on microbial identification methods in the biopharma industry. Tirumalai explained that analytical techniques can be appropriate in specific scenarios. For example, a microbial identification system may require precise identification at the genus or even strain […]
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mRNA Vaccine Quality Control with Nanopore Sequencing

Helen Gunter from The BASE Facility of The University of Queensland in Australia presented at the ONT Biopharma Day. The session’s title was “Using nanopore sequencing for mRNA vaccine quality control: a journey from R&D to GMP.” They noted that since the COVID vaccines, there has been interest in synthetic mRNAs. Gunter spoke about the […]
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Innovations in Genetic Testing: The CRDC’s Impact

Wanqing Shao from Boston Children’s Hospital presented at the Nanopore Community Meeting on “Maximizing the power of genomic sequencing in pediatric rare disease.” Shao is a Genomics Scientist working in a collaborative team: the Children’s Rare Disease Collaborative (CRDC). They are interested in rare and complex diseases that are often misdiagnosed. Shao also noted that […]
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Understanding Long-Read Phasing Methods in Genomics

Nikhita Damaraju from the University of Washington in Seattle presented at the Nanopore Community Meeting in Boston. The session’s title was “Evaluating the quality of long-read phasing methods in clinically relevant genes.” They explained that variants are inherited in equal measure. Damaraju defined phasing as the process of assigning variants to parental copies. Understanding which […]
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Understanding the NO-MISS Workflow for Microbial Sequencing

Tonight, I watched the second half of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” Different extraction methods produced varying yields. Bead-beating and enzymatic lysis extractions affect read length and throughput, while fungal samples produce lower yields. Some potential issues include incomplete lysis and contaminants. Additional clean-up […]
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DNA Extraction Techniques: Bead Beating vs Enzymatic Lysis

We hosted a bioinformatics workshop with Oxford Nanopore Technologies specialists! It was fun to learn about EPI2ME updates. Tonight, I watched the beginning of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” The webinar began with a review of the applications related to microbial isolate […]
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Unlocking Genomic Frontiers: ONT’s Long-Read Sequencing Breakthroughs

Tonight I started watching a longer session from Oxford Nanopore Technologies (ONT) summer programming. “The era of complete genomes, at any scale” was the title of a session Cora Vaher gave. They described how long-read sequencing with ONT has changed sequencing. Vaher stated that “the era of full genomes is coming” because there are many […]
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Nanopore Sequencing: Experiment Planning and Workflow Breakdown

As we prepare for the start of a new semester, I decided to watch a Masterclass from London Calling 2024. Akelia Wauchope-Odumbo, Associate Director Technical Applications—Americas with Oxford Nanopore Technologies, presented on experimental design. The session title was “How to get started with nanopore sequencing and plan your experiment.” The masterclass series guides users from “sample to […]
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Shifting to Oxford Nanopore for Clinical 16S rRNA Gene Sequencing

Trish Simner is the Director of Bacteriology and Infectious Disease Sequencing Laboratories. They work at the Johns Hopkins University School of Medicine in Baltimore, Maryland. They presented at London Calling 2024. The session’s title was “Bringing Nanopore Sequencing into the Clinical Microbiology Setting with Targeted Approaches.” Simner spoke about the advantages of long reads and […]
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