PromethION

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Improved DNA and RNA Sequencing with R10.4.1 and Duplex Reads

Miten Jain from Northeastern University presented at London Calling 2023 about “A complete analysis of human genomes and transcriptomes using nanopore sequencing.” They spoke about R10.4.1 ultralong DNA sequencing. Jain’s group has been using this sample prep and flow cells with N50s between 90-110 kb and throughput of >100 Gb per PromethION flow cell. The […]
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Combined Transcriptome and Epitranscriptome in the Human Brain

Josie Gleeson from The University of Melbourne in Australia presented at London Calling 2023 about “Integrating the transcriptome and epitranscriptome of the human brain using direct RNA sequencing.” They spoke about the advantages of direct RNA sequencing with Oxford Nanopore Technologies: you obtain splicing patterns, isoform quantification, polyA tail length, modified nucleotides, and novel exon/intron […]
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The Genomics England Long-read Sequencing Program

I am at the Gordon Research Conference in Maine. This is my first GRC! I was able to rest a little this afternoon and watched the London Calling 2023 session entitled “Genomics England long-read cancer whole -genome sequencing pilot” presented by Helen Webb from Genomics England, UK. Genomics England is also called GEL. Webb disclosed […]
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Epigenetic Aging Clock for Cattle

Loan to Nguyen from The University of Queensland, Australia presented at the Nanopore Community Meeting 2021 on “An epigenetic aging clock for cattle using portable sequencing technology.” They spoke about the need learn birth date of cattle for breed registrations and genomic selection. However, there is no gene clock for cattle. Thus, they took sixty […]
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Raw Read Accuracies and Genome Assembly

Alexander Wittenberg from KeyGene in the Netherlands presented at the Nanopore Community Meeting 2021 on “Accuracy improvements in crop genome assembly using the Q20+ chemistry.” They presented on improvements in sequencing and crop assembly using Q20+ chemistry. KeyGene is using genome assembly and the early access ONT program to identify pan-genome and variants. KeyGene has […]
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Allele-specific Methylation in Fishes

Henri van Kruistum presented at the Nanopore Community Meeting 2021 on “Allele-specific DNA methylation in the live-bearing fish Poeciliopsis gracilis.” Kruistum is a Ph.D. student at Wageningen University in the Netherlands. They are working on the Poecillidae live-bearing fishes. There are sever species and on the genomic level there is interest in learning about the […]
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