January 2023 – Page 2 – Carlos C. Goller

January 21, 2023
By Carlos C. Goller
Oxford Nanopore Technologies, Publishing, Reflections, sequencing, Undergraduate Research, Website

Planning Nanopore Sequencing Experiments

Anupama Chandramouli presented the NCM 2022 Oxford Nanopore Technologies masterclass entitled “How to get started with nanopore sequencing and plan your experiment.” Chandramouli is a Field Application Scientist for the North East Region, Americas with Oxford Nanopore Technologies. They described the process starting with extraction of nucleic acids. Chandramouli explained that the nanopores they use […]

January 20, 2023
By Carlos C. Goller
Delftia, Presentations & Talks, Reflections, sequencing, Softwares, Undergraduate Research, Website, Why, Workshop

Alignments and Assemblies

We began working with the VolTRAX2 today. I’m excited to use it for library preps along with the Miroculus Miro. Tonight I watched the NCM 2022 Masterclass session by Matt Attreed on “How to generate and call variants.” Attreed is a technical applications scientist (bioinformatics) with Oxford Nanopore Technologies. They began by providing an introduction […]

January 19, 2023
By Carlos C. Goller
Molecular Biology Education Research, Oxford Nanopore Technologies, Reflections, sequencing, Softwares

Basecalling for Modified Bases

The Oxford Nanopore Technologies (ONT) NCM 2022 masterclass I watched tonight was “How to basecall your data and detect methylation” with Jessica Anderson, a field application scientist with ONT. The recording is available. Anderson described how Nanopore sequencing works: as DNA/RNA strands pass through the pore, changes in current are detected. Anderson explained that basecalling […]

January 18, 2023
By Carlos C. Goller
Delftia, Oxford Nanopore Technologies, Presentations & Talks, Reflections, sequencing, Softwares, Undergraduate Research, Workshop

Flongles and Several MinIONs and PromethIONs

Austin Compton is a Technical Applications Specialist at Oxford Nanopore Technologies and presented a masterclass at NCM 2022. We’ve used the MinION but I don’t know as much about the PromethION and Flongle. Compton shared a slide that indicated that the Flongle flow cells have 126 pores, MinION flow cells have 512, and PromethION flow […]

January 17, 2023
By Carlos C. Goller
Delftia, Oxford Nanopore Technologies, Presentations & Talks, Reflections, Softwares, Undergraduate Research, Website, Workshop

A Collection of Library Prep Options for Nanopore

Yan Yang from the Oxford Nanopore Technologies Technical Services North America West Coast Team, presented the NCM 2022 Masterclass “How to select the right library preparation workflow for your experiment.” Yang explained how Nanopore DNA/RNA sequencing works as the nucleic acid is passed through a nanopore and electrical signals are interpreted into sequence data. Yang […]

January 16, 2023
By Carlos C. Goller
Metagenomics, Oxford Nanopore Technologies, Reflections, sequencing, Undergraduate Research, Website

Considerations for High-quality DNA and RNA for Oxford Nanopore Sequencing

Tonight I started watching Oxford Nanopore Technologies NCM 2022 Masterclasses. The first recording I watched was by Vania Costa on “How to extract high-quality DNA and RNA.” They spoke about considerations for different fragment lengths. For example, you may want long and ultra-long DNA fragments for assembly. However, for cell-free DNA sequencing you are likely […]

January 15, 2023
By Carlos C. Goller
Academic Development, Accessibility and UDL, Constructivism, Course Design, Discussions, Online Learning, Presentations & Talks, QM, Reflections, UDL

From Course Preparation to QM Review

Tonight I watched the Quality Matters session entitled “Two Sides of the QM Equation: Perspectives from a Course Representative and Course Reviewer” presented by Cindy Ambrose (Low Country Virtual), Jenna Wells (Berkley County School District), and Sharon McMahon (Beaufort County School District). They shared their experience submitting and reviewing courses. The first part of the […]

January 14, 2023
By Carlos C. Goller
Academic Development, Accessibility and UDL, Constructivism, Course Design, Discussions, Instructor Behaviors, Online Learning, Pedagogy, Presentations & Talks, Reflections

CHLOE 7

Tonight I watched the QM session entitled “CHLOE 7: Tracking Online Learning from Mainstream Acceptance to Universal Adoption. CHLOE stands for The Changing Landscape of Online Education. Richard Garnett, Eduventures Chief Research Officer at Encoura, was the first speaker. Dr. Bethany Simunich, Director of Research and Innovation at Quality Matters was the co-presenter. Over the […]

January 13, 2023
By Carlos C. Goller
Academic Development, Accessibility and UDL, Constructivism, Course Design, Online Learning, Pedagogy, QM, Reflections

Fifty QM Course Reviews

Paula McDougald from Virtual Arkansas and Dr. Brandie Benton (Virtual Arkansas) presented a QM session I watched tonight entitled “My Circus and My Monkeys: Sharing What We’ve Learned Through Simultaneous QM Reviews.” Virtual Arkansas is funded through the Department of Education to provide supplemental instruction in the state. They have 130 teachers and students from […]

January 12, 2023
By Carlos C. Goller
Academic Development, Accessibility and UDL, Course Design, Instructor Behaviors, Online Learning, QM, Reflections, Why

Supporting Teachers to Create Better Learning Experiences Online

Tonight I watched the QM recording for the session entitled “Student Connection and Rapport in the Virtual Environment,” presented by Lisa Pizzuto, Program Specialist/Coordinator at Fulton County Schools. Pizzuto has been teaching online for seventeen years! Pizzuto’s objective was to share ideas for how we can personalize a generic course and develop connections with students. […]

Month: January 2023