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Sequencing and Assembly of an Endangered Tortoise Genome

Tonight I watched the Oxford Nanopore Technologies (ONT) London Calling 2023 Showcase Stage on Conservation. In this session, Luca Pandolfini from the Italian Institute of Technology in Italy presented “From genome assembly to epigenome characterisation: a nanopore journey in the footsteps of an endangered tortoise.” Pyxis arachnoides is a tiny tortoise that is critically endangered […]
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Melon Genome Sequencing and the NLRome

Javier Belinchon-Moreno from INRAE-EPGV in France spoke at London Calling 2023 about “Exploring the genetic and functional diversity of the NLRome/resistome in melon using nanopore adaptive sampling.” They started by describing how Nanopore adaptive sampling works and its adaptability to a variety of uses. Belinchon-Moreno and team used Nanopore adaptive sampling (NAS) to “decipher the […]
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Genome Assemblies for Chromosomal Evolution Analyses of Marsupials

Patrick G.S. Grady from the University of Connecticut presented at London Calling 2023 a session entitled: “Unraveling chromosomal evolution in marsupials: comparative genomics and methylomics with telomere-to-telomere precision.” Grady spoke about the use of marsupials as a model. They have pentapedal locomotion, unique reproduction, and dynamic forms of cancer. Their genome scan be studied with […]
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CanMAGs from Long-read Assemblies

Anna Cusco from Vetgenomics in Spain spoke at the 2021 Nanopore Community Meeting about “Long-read metagenomics and Hi-C proximity ligation to characterize a canine fecal microbiome.” They have been characterizing microbiomes using metagenomics and Hi-C. The (now) classical approach is amplicon sequencing; however, it allows for taxonomic classification but not everything can be detected. With […]
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Raw Read Accuracies and Genome Assembly

Alexander Wittenberg from KeyGene in the Netherlands presented at the Nanopore Community Meeting 2021 on “Accuracy improvements in crop genome assembly using the Q20+ chemistry.” They presented on improvements in sequencing and crop assembly using Q20+ chemistry. KeyGene is using genome assembly and the early access ONT program to identify pan-genome and variants. KeyGene has […]
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Org.one Collaborations for Sequencing Biodiversity

Tomas Marques-Bonet from the Institute of Evolutionary Biology in Spain presented at the Nanopore Community Meeting 2021 about “Org.one: a new program to promote sequencing biodiversity.” Tomorrow London Calling 2023 begins and I am continuing to watch previous sessions. Marques-Bonet highlighted how biodiversity is at risk, with more than 37,400 species threatened with extinction. Amphibians […]
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Liftoff Annotation of Novel Genes from T2T Human Genome

Alaina Shumate from John Hopkins University presented at the Nanopore Community Meeting 2021 about “The annotation of novel genes in a complete human genome.” They began by describing how in 2003 scientists “finished” the Human genome Project but there still was missing sequence! In 2021, the Telomere-to-Telomere (T2T) Consortium actually completed the sequence of a […]
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