DNA extraction

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DNA Extraction Techniques: Bead Beating vs Enzymatic Lysis

We hosted a bioinformatics workshop with Oxford Nanopore Technologies specialists! It was fun to learn about EPI2ME updates. Tonight, I watched the beginning of a new Knowledge Exchange session focusing on “Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow.” The webinar began with a review of the applications related to microbial isolate […]
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Improved Plant Genome Assembly with Ultra-Long Nanopore Sequencing

Bosheng Li from the Institute of Advanced Agricultural Sciences of Peking University in China presented at London Calling 2024. The session’s title was “Plant T2T genome assembly using ultra-long and adaptive nanopore sequencing.” They spoke about the importance of ultra-long reads. These reads produce N50 length >100 kb. These are crucial in the assembly of […]
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Nanopore Sequencing Advancements: Optimizing Metagenome Assemblies

It was great to see Lauren Lui from the Lawrence Berkley National Laboratory present at London Calling 2024 on “An unprecedented metagenomic landscape of European and North American coastal samples.” Lui is a research scientist and, while at Miten Jain’s lab at Northeastern University, loaded 34 PromethION flow cells, generating 5 Tbases of data! Lui […]
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Portable Lab for Wastewater DNA Extraction

Tonight, I started watching sessions from London Calling 2024! Rea Kobialka from the Institute of Animal Hygiene and Veterinary Public Health in Germany presented a session entitled “Mobile lab for rapid detection of pathogens in wastewater in sub-Saharan Africa.” They emphasized the importance of surveillance. The research team developed a portable suitcase mobile lab. The […]
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Sequencing on the Farm

Charles C. Kayuki from the Tanzania Agricultural Research Institute in Mikocheni, Tanzania, presented at London Calling 2019 on “Portable DNA sequencing in remote East African farms.” They are using genomics to fight plant pathogens, focusing on cassava. Kayuki explained that it is an easy crop to grow and drought-resistant. It is susceptible to two important […]
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ONT Masterclass 2023 for DNA/RNA Extraction

Tonight I watched a London Calling 2023 Masterclass on “How to extract high-quality DNA and RNA” with Vania Costa, a Field Applications Scientist with Oxford Nanopore Technologies. This is part of a series of Masterclasses. The learning objectives, Costa explained, were how to determine the right extraction method as well as common practices. Costa said […]
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You can’t sequence what you don’t have!

Tonight I continued watching masterclass sessions from the 2022 London Calling event. Vania Costa is an Applications Scientist with Oxford Nanopore Technologies and presented a masterclass on “Preparation: extracting high-quality DNA and RNA.” They spoke about pre-lab considerations, sample storage, DNA and RNA extraction, sample quality control, and several case studies. Pre-lab considerations mentioned by […]
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