EPI2ME

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Fieldwork to Study Mangrome Microbial Diversity

Felipe Baez Aguirre from the University of the Andes in Colombia presented a session entitled “On-site nanopore sequencing reveals microbial diversity of a Colombian Pacific Coast mangrove soils” at the Nanopore Community Meeting in Houston. Baez Aguirre spoke about the location of the studies. They had limited access to electricity in the Bahia Malaga. The […]
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Data Analysis Options with ONT

Tonight I watched the Nanopore Community Meeting Houston session by Bryant Catano, ONT Product Specialist. The session was a Masterclass entitled “How to get started with data analysis.” Catano spoke about the variety of options for data analysis available, from point-and-click to command line. Tools are available that are accessible, scalable, and versatile. Catano noted […]
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Structural Variant Detection and Analysis

Continuing with the Nanopore Learning course on Human Genome Sequencing and Analysis, tonight I watched the video about structural variation detection. Anthony Doran, a member of the Technical Services Team with Oxford Nanopore Technologies, defined structural variation as large structural changes in your sample compared to the reference genome. The examples Doran shared on a […]
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The EPI2ME Labs wf-human-variation Workflow

Happy Thanksgiving! Tonight I watched a second video on EPI2ME Labs that is part of the Human genome sequencing and analysis course offered by Nanopore Learning. This half-hour video presented by Bala Periaswamy from Oxford Nanopore spoke about EPI2ME Labs and how it can run workflows through the use of Docker and Nextflow. The wf-human-variation […]
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Data Analysis Solutions from ONT

The next lesson in the Nanopore Learning course for Human genome sequencing and analysis I watched was the introduction to data analysis solutions video. The speaker, Anthony Doran, shared a graphic with the different bioinformatics options provided by Oxford Nanopore. EPI2ME is the real-time cloud-based platform. There are several workflows for quality control, alignment, and […]
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The Cloud-based EPI2ME Metagenomics Workflows

Tim Walker from the Technical Services Group conducted a demonstration of EPI2ME as part of the Nanopore Learning course. Walker noted that EPI2ME currently hosts free metagenomic classification pipelines including WIMP, the antimicrobial resistance workflow, and the Fastq 16S workflow. The 16S workflow works with the 16S barcoding kit. This is the kit and workflow […]
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Metagenomic Classification and ONT Reads

Tim Walker from the Technical Services Team with Oxford Nanopore Technologies presented the Nanopore Learning Course – Metagenomics lesson I watched tonight. They spoke about metagenomic classification techniques to identify organisms from sequence data and annotate genes. For targeted metagenomics, ITS, 16S, or antibiotic resistance genes can be amplified and sequenced. Whole genome sequencing can […]
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PCR-based ONT Kits and 16S Analysis

Tonight I continued watching the Nanopore Learning Metagenomics series. The lessons focused on Library Preparation: Low input PCR kits. Hazel Johnson spoke about low-input kits. Library preparation involves attachment of sequencing adapters that are “Y” shaped and include a motor protein and leader sequence. A tether site facilitates the capture of the molecule. When you […]
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