Eva Maria Novoa from the Centre for Genomic Regulation in Spain presented at London Calling 2025 on “Decoding the epitranscriptome at single-molecule resolution: towards clinical applications.” Novoa spoke about ribosomal RNA modifications. They study ribosomal RNA modifications. There are more than 12 modifications in rRNA. The new generation of algorithms allows the detection of more […]
Yasuhiro Murakawa from Kyoto University in Japan presented at London Calling 2025 on “A compendium of human RNA structures and modifications.” Kazuhiro Takeuchi from the Murakawa lab generated a de novo human transcriptome assembly using “full-length” cDNA sequencing. Murakawa noted that there are many RNA forms and regulation events. Long-read sequencing methods, while useful in […]
Logan Mulroney from the EMBL-EBI in the UK and Italy spoke at the Nanopore Community Meeting in Boston. The title of the session was “A survey of RNA modifications across the human transcriptome by direct RNA nanopore sequencing.” They spoke about RNA modification and their implications in diseases such as cancer. RNA modifications can have […]
The London Calling 2024 virtual platform is no longer available! So, tonight, I started watching the available YouTube playlists. The first consists of studio interviews. I watched the “LC24 Studio: Understanding Structural Variation, Population Genomics” session. Zoe McDougall interviewed presenters. Belen de la Morena from the University of Murcia in Spain spoke about their interest […]
Tommaso Leonardi from the Italian Institute of Technology (IIT) presented a lightning talk at London Calling 2019 on “signal level RNA modifications detection in eukaryotic non-coding RNAs.” Leonardi explained that there are many RNA modifications, and they developed the tool Nanocompore to compare signals from two conditions. It also takes into account replicates. Nanopore also […]
Tonight, I watched the Nanopore Learning Knowledge Exchange about Direct RNA Sequencing. Libby Snell and Botond Sipos explained how the kit and process work. Snell began by addressing the question: why use direct RNA sequencing. They explained that it is truly native RNA sequencing since the RNA strand is read directly in the Nanopore. There […]
Tsutomu Suzuki from the University of Tokyo in Japan presented at London Calling 2023 a fifteen-minute recorded session about “Nanopore sequencing of tRNA modifications.” Suzuki is a biochemist in the field of RNA biology. They began describing how RNA modification enriches the diversity of RNA. Suzuki noted that tRNA modifications have been studied for a […]
Jade Forster from the University of Southampton in the UK presented at London Calling 2023 about “Identifying m6A RNA modifications in neuroblastoma cell lines using nanopore sequencing.” This session was timely as we are doing our first direct RNA sequencing experiment and GridION run! Forster spoke about epitranscriptomics and RNA modifications. They noted that the […]
We have been trying bacterial transcriptomics using Nanopore sequencing. I am particularly interested in direct RNA sequencing. Tonight I watched the London Calling 2023 session by Libby Snell, Principal Scientists/Applications at Oxford Nanopore Technologies, and Martin Smith from Sainte-Justine University Hospital Research Center in Canada. They presented a session entitled “Direct RNA sequencing update (SQK-RNA004): […]
Ewan Birney from EMBL & EMBL-EBI in the UK presented at the Nanopore Community Meeting 2021 on “DNA and RNA modifications: how nanopore is giving us new biological insights -from COVID to gene expression.” They began by explaining that nanopore sequencing and sensing modification was recognized as feasible in 2014. DNA modifications such as CpG […]