sequencing

Optimizing QC Testing of mRNA Vaccines and Therapeutics: Lonza and ONT Collaboration Overview

Tonight, I watched the London Calling 2024 session on “Optimizing QC testing of mRNA vaccines and therapeutics.” Telmo Graca from Lonza, Switzerland, was the speaker for this session. They had learning objectives for the ten-minute talk. The three learning objectives focused on providing an overview of the requirements for mRNA therapeutics, the collaboration process between […]
Read more

The Future of Clinical Genetic Testing with Long-Read Sequencing

Danny E. Miller from the University of Washington spoke at London Calling on the clinical applications of long-read sequencing. The title of this session was “Long-read sequencing as the future of clinical genetic testing.” Miller started with three points. The first was that long-read sequencing will change clinical testing within five years. The second reason […]
Read more

Oxford Nanopore’s Modified Base Research: Latest Advances and Future Developments

I watched the London Calling 2024 session about DNA and RNA modification calling tonight. Adrien Leger, Director of Modified Base Research at Oxford Nanopore Technologies, was the presenter. They explained how the research team trains a modified basecaller using synthetic oligos. There is primary and secondary validation with known oligos. Accuracy and several other metrics […]
Read more

Uncovering ‘Dark’ Genomic Regions with Nanopore Sequencing – Mayo Clinic Insights

Devin Oglesbee from the Mayo Clinic spoke about “Nanopore sequencing medically relevant ‘dark’ genomic regions” at London Calling 2024. The Mayo Clinic has several locations and is interested in learning about dark/camouflaged genomic regions that are “not being met in the clinical laboratory.” Oglesbee spoke about the difference between “dark by depth” and “dark by […]
Read more

Methylomics and Fragmentomics: Pioneering Cancer Detection Models

Hanlee Ji from Stanford University presented at London Calling 2024 on “Genomic sequencing for characterizing tumor minimal residual disease versus early cancer.” Ji spoke about apoptosis and circulating tumor DNA. Ji and the team evaluated the recurrence of metastatic colorectal cancer with circulating DNA monitoring. Early detection of breast cancer can benefit from nanopore sequencing […]
Read more

Real-Time Analysis Challenges and RT-Metagenomics Workflow Demo

Tonight, I watched the London Calling 2024 EPI2ME Product Demo. The session title is: “EPI2ME: democratising bioinformatics – from point-and-click analysis to custom integrations.” Sarah Griffiths, ONT Bioinformatics Workflow Developer, gave an overview of the EPI2ME workflows. The workflows use Nexflow and containers. At the time of recording, they had seventeen workflows. Griffiths noted that […]
Read more

Nanopore Sequencing for mRNA Vaccine Quality Assurance

Helen Gunter from the BASE mRNA Facility at the University of Queensland in Australia spoke at London Calling 2024 on “Innovations in mRNA vaccine precision manufacturing.” BASE is the largest mRNA manufacturer in Australia, supplying commercial, government, and academic research labs. The plasmid is amplified, linearized, and used as a template to transcribe the mRNA. […]
Read more

Discovering Deeply Intronic Mutations with Nanopore Sequencing

Tom Walsh from the University of Washington in the USA spoke at London Calling about “Discovery of deeply intronic damaging mutations with nanopore sequencing.” Walsh explained that in the 1990s, genetic testing for predisposition to breast cancer began. In the 200s, additional cancer-risk genes were discovered. Now, there are numerous panels and ten genes for […]
Read more

Nanopore Sequencing Advancements: Optimizing Metagenome Assemblies

It was great to see Lauren Lui from the Lawrence Berkley National Laboratory present at London Calling 2024 on “An unprecedented metagenomic landscape of European and North American coastal samples.” Lui is a research scientist and, while at Miten Jain’s lab at Northeastern University, loaded 34 PromethION flow cells, generating 5 Tbases of data! Lui […]
Read more

Intraoperative Brain Tumor Diagnosis

“Robbing the array to feed the pore: exploring ultra-fast brain tumor diagnosis” is the title of the session Dr. Simon Paine from Nottingham University Hospitals NHS Trust in the UK presented at London Calling 2024. What a creative title! Paine is a clinical consultant and neuropathologist. Paine explained that neuropathologists are trying to predict tumor […]
Read more
Press ESC key to close search